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Improved protoplast culture and stability of cytoplasmic traits in plants regenerated from leaf protoplasts of cauliflower (Brassica oteracea ssp.botrytis)

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Abstract

Nearly 1000 plants have been regenerated from leaf protoplasts of two cauliflower (Brassica oleracea ssp.botrytis) alloplasmic inbred lines. One line (7642A) carried the Ogura (R1) cms cytoplasm derived from radish; the other line (7642B) carried a normalBrassica cytoplasm and was the fertile maintainer for the cms line. The majority of regenerated plants displayed normal vegetative morphology; they formed normal cauliflower heads and retained the floral characteristics of seed-grown plants from which they were derived. We found no change in either male sterility or in the low temperature-induced chlorosis associated with the 7642A line. Mitochondrial DNA analysis by hybridization with five cloned mtDNA probes revealed no apparent alteration in 75 regenerated plants of both lines. These results indicate that cytoplasmic traits inBrassica oleracea are stable after one cycle of in vitro culture and regeneration.

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References

  1. Aviv D, Galun E (1987) Chondriome analysis in sexual progenies ofNicotiana cybrids. Theor Appl Genet 73: 821–826

    Google Scholar 

  2. Barsby TL, Yarrow SA, Kemble RJ, Grant I (1987) The transfer of cytoplasmic male sterility to winter-type oilseed rape (Brassica napus L.) by protoplast fusion. Plant Sci 53: 243–248

    Google Scholar 

  3. Bayliss MW (1980) Chromosomal variation in plant tissues in culture. Intern Rev Cytol Suppl 11A: 113–144

    Google Scholar 

  4. Bernatzky R, Tanksley SD (1986) Majority of random cDNA clones correspond to single loci in the tomato genome. Mol Gen Genet 203: 8–14

    Google Scholar 

  5. Bidney DL, Shepard JF, Kaleikau E (1983) Regeneration of plants from mesophyll protoplasts ofBrassica oleracea. Protoplasma 117: 89–92

    Google Scholar 

  6. Chourey PS, Lloyd RE, Sharpe DZ, Isola NR (1986) Molecular analysis of hypervariability in the mitochondrial genome of tissue cultured cells of maize and sorghum. In Mantell SH, Chapman GP, Street PFS (Eds) The Chondriome-Chloroplast and Mitochondrial Genomes (pp 177–191) Longman Scientific, Harlow, UK

    Google Scholar 

  7. Dickson MH (1985) Male sterile persistent white curd cauliflower NY 7642A and its maintainer NY 7642B. Hort Science 20: 957

    Google Scholar 

  8. Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36: 715–722

    Google Scholar 

  9. Earle ED, Gracen VE, Best VM, Batts LA, Smith ME (1987) Fertile revertants from S-type male-sterile maize grown in vitro. Theor Appl Genet 74: 601–609

    Google Scholar 

  10. Foulger D, Jones MGK (1986) Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars. Plant Cell Rep 5: 72–76

    Google Scholar 

  11. Fu Y, Jia S, Lin Y (1985) Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var. ‘capitata’) Theor Appl Genet 71: 495–499

    Google Scholar 

  12. Gengenbach BG, Connelly JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59: 161–167

    Google Scholar 

  13. Glimelius K (1984) High growth rates and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61: 38–44

    Google Scholar 

  14. Hanson MR (1984) Stability, variation, and recombination in plant mitochondrial genomes via tissue culture and somatic hybridization. Oxford Surveys Plant Mol Cell Biol 1: 33–52

    Google Scholar 

  15. Hartmann C, deByuser J, Henry Y, Falconet D, Lejeune B, Benslimane A-A, Quetier F, Rode A (1987) Time course of mitochondrial genome variation in wheat embryogenic somatic tissue cultures. Plant Sci 53: 191–198

    Google Scholar 

  16. Hodges TK, Kamo KK, Imbrie CW, Becwar MR (1986) Genotype specificity of somatic embryogenesis and regeneration in maize. Bio/Technol 4: 219–223

    Google Scholar 

  17. Jourdan PS, Earle ED (1989) Genotypic variability in the frequency of plant regeneration from leaf protoplasts of fourBrassica species and ofRaphanus sátivus. J Am Soc Hort Sci (in press)

  18. Jourdan PS, Earle ED, Mutschler MA (1989) Synthesis of male sterile, triazine-resistantBrassica napus by somatic hybridization between cytoplasmic male sterileB. oleracea and atrazine-resistantB. campestris. Theor Appl Genet (in press)

  19. Keller WA, Arnison PG, Cardy JC (1987) Haploids from gametophytic cells-recent developments and future prospects. In: Green CE, Somers DA, Hackett WP, Biesboer DD (Eds) Plant Tissue and Cell Culture (pp 223–241) Alan R Liss, NY

    Google Scholar 

  20. Kemble RJ, Shepard JF (1984) Cytoplasmic DNA variation in a potato protoclonal population. Theor Appl Genet 69: 211–216

    Google Scholar 

  21. Kemble RJ, Yarrow SA, Wu SC, Barsby TL (1988) Absence of mitochondrial and chloroplast DNA recombinations inBrassica napus plants regenerated from proto plasts, protoplast fusions and anther culture. Theor Appl Genet 75: 875–881

    Google Scholar 

  22. Kumar A, Cocking EC (1987) Protoplast fusion: a novel approach to organelle genetics in higher plants. Am J Bot 74:1289–1303

    Google Scholar 

  23. Linsmaier M, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18: 100–127

    Google Scholar 

  24. Lu DY, Pental D, Cocking EC (1982) Plant regeneration from seedling cotyledon protoplasts. Z Pflanzenphysiol 107:59–63

    Google Scholar 

  25. McNay JW, Chourey PS, Pring DS (1984) Molecular analysis of genomic stability of mitochondrial DNA in tissue-cultured cells of maize. Theor Appl Genet 67: 433–437

    Google Scholar 

  26. Morgan A, Maliga P (1987) Rapid chloroplast segregation and recombination of mitochondrial DNA inBrassica cybrids. Mol Gen Genet 209: 240–246

    Google Scholar 

  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  28. Nagy JJ, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplasts ofNicotiana sylvestris. Z Pflanzenphysiol 78: 453–455

    Google Scholar 

  29. Ogura H (1968) Studies on the new male-sterility in Japanese radish, with special reference to the utilization of this sterility towards practical raising of hybrid seed. Mean Fac Agric Kagoshima Univ 6: 39–78

    Google Scholar 

  30. Palmer JD, Shields CR (1984) Tripartite structure ofBrassica campestris mitochondrial genome. Nature 307: 437–440

    Google Scholar 

  31. Palmer JD, Sheilds CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploidBrassica species. Theor Appl Genet 65: 181–189

    Google Scholar 

  32. Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rouselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191: 244–252

    Google Scholar 

  33. Robertson D, Earle ED (1986) Plant regeneration from leaf protoplasts ofBrassica oleracea var italica cv. ‘Green Comet’ broccoli. Plant Cell Rep 5: 61–64

    Google Scholar 

  34. Rode A, Hartmann C, Dron M, Picard E, Quetier F (1985) Organelle genome stability in anther-derived doubled haploids of wheat (Triticum aestivum L. cv. ‘Moisson’) Theor Appl Genet 71: 320–324

    Google Scholar 

  35. Rode A, Hartmann C, Falconet D, Lejeune B, Quétier F, Benslimane A, Henry Y, deBuyser J (1987) Extensive mitochondrial DNA variation in somatic tissue cultures initiat ed from wheat immature embryos. Curr Genet 12: 369–376.

    Google Scholar 

  36. Schenck HR (1984) Isolation and culture of protoplasts:Brassica. In: Vasil I (Ed) Cell Culture and Somatic Cell Genetics of Plants. Vol 1 (pp 356–369) Academic Press, New York

    Google Scholar 

  37. Schenck HR, Röbbelen G (1982) Somatic hybrids by fusion of protoplasts fromBrassica oleracea andB. campestris. Z Pflanzensucht 89: 278–288

    Google Scholar 

  38. Shirzagedan M, Christey M, Earle ED, Palmer JD (1989) Rearrangement, amplification and assortment of mitochondrial DNA molecules in cultured cells ofBrassica campestris. Theor Appl Genet (in press)

  39. Southern EM (1975) Detection of specific sequencies among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

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Author notes

  1. P. S. Jourdan

    Present address: Department of Horticulture, The Ohio State University, 2001 Fyffe Ct, 43210-1096, Columbus, OH, USA

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, USA

    P. S. Jourdan, E. D. Earle & M. A. Mutschler

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  1. P. S. Jourdan
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  2. E. D. Earle
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  3. M. A. Mutschler
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Jourdan, P.S., Earle, E.D. & Mutschler, M.A. Improved protoplast culture and stability of cytoplasmic traits in plants regenerated from leaf protoplasts of cauliflower (Brassica oteracea ssp.botrytis). Plant Cell Tiss Organ Cult 21, 227–236 (1990). https://doi.org/10.1007/BF00047615

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  • DOI: https://doi.org/10.1007/BF00047615

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