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Nitro musk compounds genotoxic activity

Genotoxicity testing of nitro musks with the SOS-chromotest and the sister-chromatid exchange test

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Abstracts

Five nitro musk compounds are widely used as fragrance ingredients in perfumes, lotions and detergents; as food additives in cigarettes and fish baits, and in such technical products as herbicide formulations and explosives. Several studies identified nitro musk compounds in aquatic environment samples, human milk and fat samples as highly lipophilic and persistent bioaccumulating environmental pollutants. To examine the compounds for genotoxic activity, musk xylene (1-tert.-butyl-3, 5-dimethyl-2, 4, 6-trinitrobenzene), musk ketone (4-tert.-butyl-3, 5-dinitro-2, 6-dimethylacetophenone), musk ambrette (l-tert.-butyl-4-methyl-6-methoxy-3, 5-dinitrobenzene), musk moskene (l, 1, 3, 3, 5-pemamethyl-4, 6-di-nitroindane) and musk tibetene (1-tert.-butyl-3, 4, 5-trimethyl-2, 6-dinitrobenzene) were tested for SOS inducing potency in the SOS chromotest with E. coli PQ37 and for sister-chromatid exchange inducing activities in human lymphocytes in vitro both in the presence and absence of an exogenous metabolizing system from rat liver S9-Mix. Nitro musks revealed no genotoxicity either in the SOS chromotest with E. coli PQ37 or in the sister-chromatid exchange test with human lymphocytes.

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References

  1. Opdyke, D.L.: Musk ambrette. Food Cosm. Toxicol. 13 (1975) 875–876

    Article  Google Scholar 

  2. Opdyke, D.L.: Musk ketone. Food Cosm. Toxicol. 13 (1975) 877–878

    Article  Google Scholar 

  3. Opdyke, D.L.: Musk tibetene. Food Cosm. Toxicol. 13 (1975) 879

    Article  Google Scholar 

  4. Opdyke, D.L.: Musk xylene. Food Cosm. Toxicol. 13 (1975) 881

    Article  Google Scholar 

  5. Opdyke, D.L.: l, l, 3, 3, 5-Pentamethyl-4, 6-dinitroindane. Food Cosm. Toxicol. 13 (1975) 885

    Article  Google Scholar 

  6. Yamagishi, T.;Miyazaki, T.;Horii, S.;Akiyama, K.: Synthetic musk residues in Biota and water from Tama River and Tokyo Bay (Japan). Arch. Environ. Contam. Toxicol. 12 (1983) 83–89

    Article  CAS  Google Scholar 

  7. Liebl, B.;Ehrensdorfer, S.: Nitromoschusverbindungen in der Frauenmilch. Gesundh.- Wes. 55 (1993) 527–532

    CAS  Google Scholar 

  8. Rimkus, G.;Wolf, M.: Nachweis von Nitromoschusverbindungen in Frauenmilch und Humanfett. Deut. Lebensm.-Rdschau. 89. Jahrg. 4 (1993) 103–107

    Google Scholar 

  9. Rimkus, G.;Wolf, M.: Rückstände und Verunreinigungen in Fischen aus Aquakultur. Deut. Lebensm. -Rdschau. 89. Jahrg. 6 (1993) 171–175

    Google Scholar 

  10. Quillardet, P.;Hofnung, M.: The SOS chromotest, a colorimetric bacterial assay for genotoxins: procedures. Mutat. Res. 147 (1985) 65–78

    CAS  Google Scholar 

  11. Hofnung, M.;Quillardet, P.: The SOS chromotest, a colorimetric assay on the primary cellular responses to genotoxic agents. Ann. NY Acad. Sci. 534 (1989) 817–825

    Article  Google Scholar 

  12. Hofnung, M.;Quillardet, P.;Görlich, O.;Touati, E.: SOS chromotest and the use of bacteria for the detection and diagnosis of genotoxic agents. In:Jolles, G.;Cordier, A. New trends in genetic risk assessment. Academic Press, London, (1989) 125–136

    Google Scholar 

  13. Mersch-Sundermann, V.;Kevekordes, S.;Mochayedi, S.: Sources of variability on the Escherichia coli PQ37 genotoxicity assay. Mutat. Res. 252 (1991) 51–60

    CAS  Google Scholar 

  14. Mersch-Sundermann, V.;Mochayedi, S.;Kevekordes, S.: Genotoxicity of polycyclic aromatic hydrocarbons inEscherichia coli PQ37. Mutat. Res. 278 (1992) 1–9

    Article  CAS  Google Scholar 

  15. Mersch-Sundermann, V.;Wintermann, F.;Kern, S.: Influence of S9-mix composition on the SOS response inEscherichia coli PQ37. Mutat. Res. 291 (1993) 53–60

    CAS  Google Scholar 

  16. Mersch-Sundermann, V.;Mochayedi, S.;Kevekordes, S.;Kern, S.;Wintermann, F.: The Genotoxicity of unsubstituted and nitrated polycyclic aromatic hydrocarbons. Anticancer Res 13 (1993) 2037–2044

    CAS  Google Scholar 

  17. Mersch-Sundermann, V.;Hofmeister, A.;Müller, G.;Hof, H.: Untersuchungen zur Mutagenität organischer Mikrokontaminationen in der Umwelt, III. Mitteilung: Die Mutagenität ausgewählter Herbizide und Insektizide im SOS chromotest (Makroverfahren). Zbl. Bakt. Hyg., I. Abt. Orig. B 189/2 (1989) 135–146

    Google Scholar 

  18. Mersch-Sundermann, V.;Müller, G.;Hofmeister, A.: Untersuchungen zur Mutagenität organischer Mikrokontaminationen in der Umwelt, IV. Mitteilung: Die Mutagenität leichtflüchtiger Organohalogene im SOS chromotest (Makroverfahren). Zbl. Bakt. Hyg., I. Abt. Orig. B 189 (1989) 266–271

    CAS  Google Scholar 

  19. Perry, P.;Wolff, S.: New giemsa method for the differential straining of sister chromatids. Nature 251 (1974) 156

    Article  CAS  Google Scholar 

  20. Zakharov, A.F.;Egolina, N.A.: Differential spiralization along mammalian mitotic chromosomes I. BRDU-revealed differentiation in Chinese hamster chromosomes. Chromosoma 38 (1972) 341

    Article  CAS  Google Scholar 

  21. Latt, S.A.: Microfluorometric detection of desoxyribonucleid acid replication in human metaphase chromosomes. Proc. Natl. Acad. Sci. 70 (1973) 3395

    Article  CAS  Google Scholar 

  22. Lamberti, L.;Brigatti Ponzetto, P.;Ardito, G.: Cell kinetics and sister-chromatid exchange frequency in human lymphocytes. Mutat. Res. 102 (1983) 193

    Article  Google Scholar 

  23. Emig, M.;Reinhardt, A.;Mersch-Sundermann, V.: A comparative study of five nitro musk compounds for genotoxicity in the SOS chromotest and Salmonella mutagenicity. Toxicology Letters 85 (1996)151–156

    Article  CAS  Google Scholar 

  24. Mersch-Sundermann, V.;Reinhardt, A.;Emig, M.: Untersuchungen zur Mutagenität, Genotoxicität und Kogenotoxicität umweltrelevanter Nitromoschusverbindungen. Zbl. Hyg. 198 (1996) 429–442

    CAS  Google Scholar 

  25. Wild, D.;King, M.T.;Gocke, E.;Eckhardt, K.: Study of artificial flavouring substances for mutagenicity in theSalmonella/Microsome, Basic and Micronucleus Tests. Fd. Chem. Toxic. Vol. 21 (1983) 6: 707–719

    Article  CAS  Google Scholar 

  26. Nair, J.;Ohshima, H.;Malaveille, C.;Friesen, M.;O’Neill, K.;Hautefeulle, A.;Bartsch, H.: Identification, occurrence and mutagenicity inSalmonella typhimurium of two synthetic nitroarenes, musk ambrette and musk xylene, in indian chewing tobacco and betel quid. Fd. Chem. Toxic. Vol 24 (1986) 1: 27–31

    Article  CAS  Google Scholar 

  27. Quillardet, P.;Hofnung, M.: The SOS chromotest: a review. Mutat. Res. 297 (1993) 235–279

    CAS  Google Scholar 

  28. Mersch-Sundermann, V.;Schneider, U.;Klopman, G.;Rosenkranz, H.S.: SOS induction inEscherichia coli and Salmonella mutagenicity: a comparison using 330 compounds. Mutagenesis, vol. 9, no. 2 (1994) 205–224

    Article  CAS  Google Scholar 

  29. Mersch-Sundermann, V.;Klopman, G.;Rosenkranz, H.S.: Chemical structure and genotoxicity: studies of the SOS chrootest. Mutat. Res. 340 (1996) 81–91

    Google Scholar 

  30. Mersch-Sundermann, V.;Emig, M.;Reinhardt, A.;Kevekordes, S.: DNA damages, micronucleus formation and P450 enzyme induction by nitro musks. Anticancer Res. 15/5A (1995) 1653–1654

    Google Scholar 

  31. Iwata, N.;Suzuki, K.;Minegishi, K.;Kawanishi, T.;Hara, S.;Endo, T.;Takahashi, A.: Induction of cytochrome P450 1A2 by musk analogues and other inducing agents in rat liver. Eur. J. Pharmacol. 248 (1993)243–250

    CAS  Google Scholar 

  32. Lehman-McKeeman, L.D.;Caudill, D.;Young, J.A.;Dierckman, T.A.: Musk xylene induces and inhibits mouse hepatic cytochrome P450 2B enzymes. Biochem. Biophys. Res. Commun., Vol. 206 (1995) 3: 975–980

    Article  CAS  Google Scholar 

  33. Maekawa, A.;Matsushima, Y.;Onodera, H.;Shibutani, M.;Ogasawara, H.;Kodama, Y.;Kurokawa, Y.;Hayashi, Y.: Longterm toxicity/carcinogenicity of musk xylol in B6C3F1 mice. Fd. Chem. Toxic. Vol. 28 (1990) 8: 581–586

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Medical Institute for General Hygiene and Environmental Health, Universität of Göttingen, Windausweg 2, D-37073, Göttingen, Germany

    Sebastian Kevekordes, Kathrin Grahl, Antonia Zaulig & Hartmut Dunkelberg

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  1. Sebastian Kevekordes
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  2. Kathrin Grahl
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  3. Antonia Zaulig
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  4. Hartmut Dunkelberg
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Kevekordes, S., Grahl, K., Zaulig, A. et al. Nitro musk compounds genotoxic activity. Environ. Sci. & Pollut. Res. 3, 189–192 (1996). https://doi.org/10.1007/BF02986953

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  • DOI: https://doi.org/10.1007/BF02986953

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