Skip to main content
Log in

Survival in the phylloplane of an introduced biocontrol agent (Trichoderma harzianum) and populations of the plant pathogenBotrytis cinerea as modified by abiotic conditions

  • Research Paper
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

Leaf populations ofTrichoderma were studied on tomato, pepper and geranium plants incubated under various conditions. Treatments involved high (>90%) or lower (75–85%) relative humidity (r.h.), temperatures of 15±3°C or 25±3°C, and soil fertilization with formulations of 2,2,5%, 3,3,8% or 5,3,8% NPK. The size of populations on leaves treated with the fungusTrichoderma harzianum differed according to plant species, leaf age, length of incubation, atmospheric conditions, and plant nutrition.T. harzianum populations were promoted in many cases by high r.h. and by 3,3,8% NPK. Interactions of introduced populations ofBotrytis cinerea with populations ofT. harzianum on tomato leaves under combinations of the above conditions showed that the population ofB. cinerea wasca tenfold lower in the presence ofT. harzianum than in the absence of this fungus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Blakeman, J.P. (1985) Ecological succession of leaf surface microorganisms in relation to biological control. pp. 6–7.in: Windels, C.E. and Lindow, S.E. [Eds.] Biological Control on the Phylloplane. American Phytopathological Society Press, St. Paul., MN.

    Google Scholar 

  2. Cutler, H.G., Severnson, R.F., Cole, P.D., Jackson, D.M. and Johnson, A.W. (1986) Secondary metabolites from higher plants, their possible role as biological control agents. p. 178.in: Green, M.B. and Hedin, P.A. [Eds.] Natural Resistance of Plants to Pests, Role of Allelochemicals,American Chemical Society Symp. No. 296.

  3. Dennis, C. and Webster, J. (1971) Antagonistic properties of species — groups ofTrichoderma. I. Production of non-volatile antibiotics.Trans. Br. Mycol. Soc. 57:25–39.

    CAS  Google Scholar 

  4. Dennis, C. and Webster, J. (1971) Antagonistic properties of species — groups ofTrichoderma. II. Production of volatile antibiotics.Trans. Br. Mycol. Soc. 57:41–48.

    Article  CAS  Google Scholar 

  5. Dik, A.J., Fokkema, N.J. and Van Pelt, J.A. (1991) Consumption of aphid honeydew, a wheat yield reduction factor, by phyllosphere yeasts under field conditions.Neth. J. Plant Pathol. 97:209–232.

    Article  Google Scholar 

  6. Dik, A.J., Fokkema, N.J. and Van Pelt, J.A. (1992) Influence of climatic and nutritional factors on yeast population dynamics in the phyllosphere of wheat.Microbiol. Ecol. 23:41–52.

    Article  Google Scholar 

  7. Elad, Y. (1990) Reasons for the delay in development of biological control of foliar pathogens.Phytoparasitica 18:99–105.

    Google Scholar 

  8. Elad, Y. (1993) Biological control of grape grey mould by means ofTrichoderma harzianum.Crop Prot. 12 (in press).

  9. Elad, Y., Chet, I. and Herds, Y. (1981) A selective medium for improving quantitative isolation ofTrichoderma spp. from soil.Phytoparasitica 9:59–67.

    Google Scholar 

  10. Elad, Y. and Cohen, A. (1991) Biological control and combination of the biocontrol agentTrichoderma with fungicides for the control of gray mold.Petria 1:148–149.

    Google Scholar 

  11. Elad, Y. and Kirshner, B. (1992) Establishment of an activeTrichoderma population in the phylloplane and its effect on grey mould (Botrytis cinerea).Phytoparasitica 20(Suppl.):137S-141S.

    Article  Google Scholar 

  12. Elad, Y. and Zimand, G. (1991) Experience in integrated chemical-biological control of grey mould (Botrytis cinerea).WPRS Bull. 14:195–199.

    Google Scholar 

  13. Elad, Y. and Zimand, G. (1992) Integrated biological chemical control of grey mould, pp. 272–276.in: Verhoeff, R., Williamson, B. and Malathrakis, N.E. [Eds.] Recent Advances in Botrytis Research.Proc. Xth Botrytis Symposium. Pudoc Scientific Publishers, Wageningen, the Netherlands.

    Google Scholar 

  14. Elad, Y., Zimand, G., Zaqs, Y., Zuriel, S. and Chet, I. (1993) Use ofTrichoderma harzianum in combination or alternation with fungicides to control cucumber grey mould (Botrytis cinerea) under commercial greenhouse conditions.Plant Pathol. 42:324–332.

    Article  CAS  Google Scholar 

  15. Fokkema, N.J. and Schippers, B. (1986) Phyllosphere versus rhizosphere as environments for saprophytic colonization. pp. 137–159.in: Fokkema, N.J. and Van den Heuvel, J. [Eds.] Microbiology of the Phyllosphere. Cambridge University Press, London.

    Google Scholar 

  16. Kritzman, G. and Netzer, D. (1978) A selective medium for isolation and identification ofBotrytis spp. from soil and onion seed.Phytoparasitica 6:3–7.

    Google Scholar 

  17. Leben, C. (1965) Epiphytic microorganisms in relation to plant disease.Annu. Rev. Phytopathol. 3:209–230.

    Article  Google Scholar 

  18. Morris, C.E. and Rouse, D.J. (1985) Role of nutrients in regulating epiphytic bacterial populations, pp. 63–82.in: Windels, C.E. and Lindow, S.E. [Eds.] Biological Control on the Phylloplane. American Phytopathological Society Press, St. Paul., MN.

    Google Scholar 

  19. Tukey, H.B. Jr. (1971) Leaching of substances from plants. pp. 67–80.in: Preece, F.F. and Dickinson, C.H. [Eds.] Ecology of Leaf Surface Micro-Organisms. Academic Press, London.

    Google Scholar 

  20. Windels, C.E. and Lindow, S.E. [Eds.] (1985) Biological Control on the Phylloplane. American Phytopathological Society Press, St. Paul., MN.

    Google Scholar 

  21. Zimand, G., Elad, Y. and Chet, I. (1991) Biological control ofBotrytis cinerea byTrichoderma spp.Phytoparasitica 19:252–253 (abstr.).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elad, Y., Kirshner, B. Survival in the phylloplane of an introduced biocontrol agent (Trichoderma harzianum) and populations of the plant pathogenBotrytis cinerea as modified by abiotic conditions. Phytoparasitica 21, 303–313 (1993). https://doi.org/10.1007/BF02981048

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981048

Key Words

Navigation