Abstract
Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage bothin vivo andin vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that bothin vitro andin vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.
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References
Abbas, A. K., Lichtman, A. H. and Pober, J. S.,Cellular and Molecular Immunology. 1st ed. (1991).
Adams, D. O. and Hamilton, T. A., The cell biology of macrophage activation.Annu. Rev. Immunol., 2, 283–318 (1984).
Akira, S., Taga, T. and Kishimoto, T., lnterleukin-6 in biology and medicine.Adv. Immunol., 54, 1–78 (1993).
Arai, K., Lee, F., Miyajima, A., Arai, N. and Yokoda, T., Cytokines; coordinators of immune and inflammatory responses.Annu. Rev. Biochem., 59, 783–836 (1990).
Balkwill, F. R. and Burke, F., The cytokine network.Immunol. Today., 10, 299–304 (1989).
Brodsky, R M. and Guagliardi, L., The cell biology of antigen processing and presentation.Ann. Rev. Immunol., 9, 707 (1994).
Chang, J. H., J. H. Cho, H. H. Kim, M. W. Lee, S. S. Han and D. I. Lee, Antitumor Astivity of Pedunculagin, One of the Ellagitannin.Arch. Pharm. Res., 18, 396–401 (1995).
Chantry, D. and Feldmann, M., The role of cytokines in autoimmunity.Biotechnol. Ther., 1, 361 (1991).
Clark, E. A. and Ledbetter, J. A., How B and T cells talk to each other.Nature, 367, 425 (1994).
Coligan, J. E., Kruisbeek, A. M., Margulies D. H., Shevach E. M. and Strober W., Current Protocols in Immunology.Wiley Interscience., 4, 2, 1 (1996).
Ezekowitz, R. A. B. and Gordon, S., Alterations in surface properties by macrophage activaton; Expression of receptors for Fc and mannose reminal glycoproteins and differentiation antigens.Contemp. Top. Immunobiol., 14, 33–56 (1984).
Hirano, T., Akira, S., Taga, T. and Kishimoto, T., Biological and clinical aspects of interleukin 6.Immunol. Today., 11, 443–449 (1990).
Joo, S. S., Han-Jun K., Hee-Seung K. and Do-Ik L., Augmentation of macrophage antitumor activities and nitric oxide production by oregonin.Archives of Pharmacal Research. 25, 457–462 (2002).
Kim, W. S., Kim, M. S., Kim, B. S., Lee, M. W., Lee, D. I., Choi, Y. W., Kim, E. J. and Kim, H. H., Preparation and Characterization of Wheat Germ Agglutinin-conjugated Praecoxin A.Yakhak Hoeji., 45, 302–309 (2001).
Lee, M. W., Tanaka, T., Nonaka, G. and Nishioka, I., Hirsunin, an ellagitannin with diarylheptanoid moiety, from Alnus hirsuta var. microphylla.Phytochemistry., 31, 967–970 (1992).
Nishioka, I., Chemistry and Biological Activities of Tannins.YakugakuZasshi., 103, 125–142 (1983).
Ogawara, M., Sone, S., and Ogura, T., Human alveolar macrophages: Wheat germ agglutinin-dependent tumor cell killing.Japanese Journal of Cancer Research (Gann)., 78, 3, 288–295 (1987).
Ogawara, M., Utsugi, M., Yamazaki, M., and Sone S., Induction of human monocyte-mediated tumor cell killing by a plant lectin, wheat germ agglutinin.Japanese Journal of Cancer Research (Gann)., 76, 11, 1107–1114 (1985).
Parker, D. C., T cell-dependent B cell activation.Ann. Rev. Immunol., 11, 331 (1993).
Roitt, I., Brostoff, J. and Male, D.,Immunology.2nd ed., 13, 25 (1989).
Romagnani, S., Cytokine production by human T cells in disease states.Ann. Rev. Immunol., 12, 227 (1994).
Sarih, M., Souvannavong, V. and Adam, D.O., Differential stimulation of macrophages for tumor cytostasis and monokine production.Cancer Letters., 64, 187–194 (1992).
Ullman, K., Northrop, J. P., Veirweij, C. L. and Crabtree, G. R., Transmission of signals from T lymphocyte antigen receptor to the gene responsible for cell proliferation and immune fuction.Ann. Rev. Immunol., 8, 421 (1989).
Van, Furth R., Current view on the mononuclear phagocyte system.Immunobiology., 161, 178–185 (1982).
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Joo, S.S., Chang, J.K., Park, J.H. et al. Immuno activation of lectin-conjugated praecoxin a on IL-6, IL-12 expression. Arch Pharm Res 25, 954–963 (2002). https://doi.org/10.1007/BF02977019
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DOI: https://doi.org/10.1007/BF02977019