Abstract
Polymeric nanoparticles composed of polystyrene (PS) as core and poly(methacrylic acid) (PMA) as corona were prepared by the dispersion copolymerization. The potential of the nanoparticles as carriers for recombinant human epidermal growth factor (EGF) was investigated. The nanoparticles showed monodispersity and good water-dispersibility. The loading content of EGF to the nanoparticles was very high due to electrostatic interaction between EGF and nanoparticles. EGF was released as a pseudo-zero order pattern after initial burst effect. The nanoparticles were sufficient for A431 cells proliferation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akashi, M., Chao, D., Yashima, E., and Miyauchi, N., Graft copolymers having hydrophobic backbone and hydrophilic branches, V. Microspheres obtained by the copolymerization of poly(ethylene glycol) macromonomer with methyl methacrylate.J. Appl. Polym. Sci., 39, 2027–2030 (1990).
Babensee, J. E., Mclntire, L. V., and Mikos, A. G., Growth factor delivery for tissue engineering.Pharm. Res., 17, 497–504 (2000).
Gill, G. N. and Lazar, C. S., Increased phosphotyrosine content and inhibition of proliferation in EGF-treated A431 cells.Nature, 293, 305–307 (1981).
Hommel, U. and Campbell, I. D., Human epidermal growth factor; high resolution solution structure and comparison with hyamn transforming growth factor a.J. Mol. Biol., 255, 8363–8365 (1992).
Jakob, M., Demarteau, O., Schafer, D., Hintermann, B., Dick, W., Beberer, M., and Martin, I., Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formatio.in vitro.J. Cell Biochem., 81, 368–377 (2001).
Mooney, D. J., Kaufmann, P. M., Sano, K., Schwendeman, S. P., Majahod, K., Schloo, B., Vacanti, J. P., and Langer, R., Localized delivery of epidermal growth factor improves the survival of transplanted hepatocytes.Biotechnol. Bioeng., 50, 422–429 (1996).
Pimentel, E., in: Handbook of growth factors: peptide growth factors. Vol.2, CRC Press, Boca Raton, FL, 1994.
Sakuma, S., Suzuki, N., Kikuchi, H., Hiwatari, K., Arikawa, K., Kishida, A., and Akashi, M., Oral peptide delivery using nanoparticles composed of novel graft copolymers having hydrophobic backbone and hydrophilic branches.Int. J. Pharm., 149, 93–106 (1997).
Sakuma, S., Suzuki, N., Sudo, R., Hiwatari, K., Kishida, A., and Akashi, M., Optimized chemical structure of nanoparticles as carriers for oral delivery of salmon calcitonin.Int. J. Pharm., 239, 185–195 (2002).
Stocum, D. L., Frontiers in medicine: regeneration.Science, 276, 59–87 (1997).
Tarnawski, A. S. and Johes, M. K., The role of epidermal growth factor (EGF) and its receptor in mucosal protection, adaptation of injury, and ulcer healing: involvement of EGF-R signal transduction pathways.J. Clin. Gastroenterol., 27, S12-S20 (1998).
Vivien, D., Galera, P., Lebrun, E., Loyau, G., and Pujol, J. P., Differential effects of transforming growth factor-beta and epidermal growth factor on the cell cycle of cultured rabbit articular chondrocytes.J. Cell Physiol., 143, 534–545 (1990).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Park, IK., Seo, SJ., Akashi, M. et al. Controlled release of epidermal growth factor (egf) from egf-loaded polymeric nanoparticles composed of polystyrene as core and poly(methacrylic acid) as coronain vitro . Arch Pharm Res 26, 649–652 (2003). https://doi.org/10.1007/BF02976715
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02976715