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A macrolide-lincosamide-streptogramin B resistance determinant gene (ermJ) cloned fromB. anthracis 590

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Abstract

Bacillus anthracis 590 having an inducible resistance determinant to MLS antibiotics was isolated from a soil sample in Korea. The resistance gene (ermJ was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment to pBS42 vector was about 2.9 kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4 kb) was determined. The DNA sequence ofermJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence ofermJ was homologous to that ofermK, the expression ofermJ is also thought to be controlled by a transcriptionl attenuation mechanism.

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Authors and Affiliations

  1. College of Pharmacy, Seoul National University, 151-742, Seoul, Korea

    Hee-Sun Kim, Eung-Chil Choi, Byong-Kak Kim & Young-In Park

  2. Department of Genetic Engineering, Korea University, 136-701, Seoul, Korea

    Hee-Sun Kim, Eung-Chil Choi, Byong-Kak Kim & Young-In Park

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  1. Hee-Sun Kim
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  2. Eung-Chil Choi
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  3. Byong-Kak Kim
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  4. Young-In Park
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Kim, HS., Choi, EC., Kim, BK. et al. A macrolide-lincosamide-streptogramin B resistance determinant gene (ermJ) cloned fromB. anthracis 590. Arch. Pharm. Res. 15, 58–61 (1992). https://doi.org/10.1007/BF02973985

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  • DOI: https://doi.org/10.1007/BF02973985

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