Skip to main content
SpringerLink
Log in

The cloning of MLS antibiotics inducible resistance gene

  • Original Articles
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

Four bacterial strains having inducible resistance to erythromycin were isolated from soil samples in Korea and characterized. MLS inducibility was checked in each strain. Cloning of inducible resistance gene(s) has been tried. Four isolates were identified asB. anthracis, Micrococcus luteus, Streptococcus faecium andB. licheniformis, in which erythromycin, oleandomycin, cirramycin and carbomycin acted as resistance inducers respectively. The resistance gene cloned fromB. licheniformis 597 strain using pBS 42 vector was found to have a 3.2 kb insert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Gryczan, T., Grandi, G., Hahn, J., Dubnau D.: Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance.Nucleic Acids Res. 8, 6081 (1980).

    Article  PubMed  CAS  Google Scholar 

  2. Horinouchi, S. and Weisblum, B.: Posttranscriptional modification of messenge RNA conformation: Mechanism of erythromycin inducible resistance.Proc. Natl. Acad. Sci. U.S.A. 77, 7079 (1981).

    Article  Google Scholar 

  3. Mayford, M. and Weisblum, B.: Messenger RNA fromStaphylococcus aureus that specifics Macrolide-Lincosamide-Streptogramin resistance: Demonstration of its conformation and of the leader peptide it encodes.J. Mol. Biol. 185, 769 (1985).

    Article  PubMed  CAS  Google Scholar 

  4. Gryczan, T., Israeli-Reches, T., Del Bue, M. and Dubnau, D.: DNA sequence and regulation of ermD, a macrolide-lincosamide-streptogramin B resistance element fromBacillus licheniformis.Mol. Gen. Genet. 194, 349 (1984).

    Article  PubMed  CAS  Google Scholar 

  5. Murphy, E.: Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant inStaphylococcus aureus.J. Bacteriol. 162, 633 (1985).

    PubMed  CAS  Google Scholar 

  6. Roberts, A.N., Hudson, G.S. and Brenner, S.: An erythromycin-resistance gene from an erythromycin-producing strain ofArthrobacter sp.Gene 35, 259 (1985).

    Article  PubMed  CAS  Google Scholar 

  7. Uchiyama, H., and Weisblum, B.: N-methyl transferase ofStreptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: Amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci.Gene 38, 103 (1985).

    Article  PubMed  CAS  Google Scholar 

  8. Monod, M., Denoya, C. and Dubnau, D.: Sequence and properties of pIM 13. A macrolidelincosamide-streptogramin B resistance plasmid fromBacillus subtilis.J. Bacteriol. 167, 138 (1986)

    PubMed  CAS  Google Scholar 

  9. Horinouchi, S., Byeon, W., and Weisblum, B.: A complex attenuator regulates inducible resistance to macrolide, lincosamide, and streptogramin B antibiotics inStreptococcus sanguis.J. Bacteriol. 154, 1252 (1983).

    PubMed  CAS  Google Scholar 

  10. Miller, J.H.:Experiments in molecular genetics, Cold Spring Harber Laboratory Press, Cold Spring Harber U.S.A. (1972).

    Google Scholar 

  11. Band, L. and Henner, D.J.:Bacillus subtilis requires a stringent Shine-Dalgarno region for gene expression. DNA,3, 17 (1984).

    PubMed  CAS  Google Scholar 

  12. Choi, E.C., Woo, J.W., and Weisblum, B.: Inducible resistance to lincosamide antibiotics by lincosamide antibiotics inBacillus licheniformis.Yakhak Hoeji 30, 317 (1986)

    CAS  Google Scholar 

  13. Cowan, S.T.:Manual for the Identification of Medical Bacteria (2nd ed.), Cambridge University Press (1974).

  14. Smeath, P.H.A., Mair, N.S., Sharpe, M.E. and Holt, J.G.Bergey's Manual of Systematic Bacteriology Vol. 2, Williams and Wilkins Co. Baltimore pp. 1599 (1986).

    Google Scholar 

  15. Buchanan, R.E. and Gibbons, N.E.:Bergey's Manual of Determinative Bacteriology, 8th ed Williams and Wilkins Company Baltimore, pp. 529 (1974).

    Google Scholar 

  16. Mandel, M. and Higa, A.: Calcium dependent bacteriophage DNA infection.J. Mol. Biol. 53, 154 (1970).

    Article  Google Scholar 

  17. Dubnau, D.:Experimental Manipulation of Gene Expression. Academic Press, New York p. 33–51 (1983).

    Google Scholar 

  18. Docherty, A., Grandi, G., Grandi, R., Gryczan, T.J., Shivakumar, A.G. and Dubnau, D.: Naturally occurring macrolide-lincosamidestreptogramin B resistance inBacillus licheniformis J. Bacteriol. 145 129 (1981).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Seoul National University, 151-742, Seoul, Korea

    Eung-Chil Choi, Kyung-Won Woo, Jong-Suk Woo, Jin-Hwan Kwak & Byong Kak Kim

Authors
  1. Eung-Chil Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyung-Won Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jong-Suk Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jin-Hwan Kwak
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Byong Kak Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, EC., Woo, KW., Woo, JS. et al. The cloning of MLS antibiotics inducible resistance gene. Arch. Pharm. Res. 12, 176–180 (1989). https://doi.org/10.1007/BF02855550

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02855550

Keywords

Search

Navigation