The intestinal microbiota are important to the host with regard to resistance they impart against bacterial infections and their involvement in mediating metabolic functions. Lactic acid producing bacteria such asLactobacillus play an important physiological role in these matters. The aim of the present study was to isolateLactobacillus sp. that inhibits enteric pathogens. Initially, 17 isolates from healthy Koreans were collected onLactobacillus selective medium. Resistance of the isolates to antibiotics including rifampicin, streptomycin, clindamycin and vancomycin was measured. One of the isolate was identified asLactobacillus ruminus on the basis of bacterial cell morphology, cultural characteristic and biochemical characteristics, 16S rRNA sequence analysis and PCR-RAPD. Antimicrobial activity of the bacterium against Vancomycin Intermediate ResistantStaphylococcus aureus (VISA) and Vancomycin-ResistantEnterococci (VRE) was measured. About 104 cells of VISA or VRE were mixed with 1, 5 and 9 mL ofL. ruminus SPM 0211 and the final volume was adjusted to 10 mL with brain heart infusion (BHI) broth. The cell suspension was incubated for 3, 6, 9 and 24 h, serially diluted and then plated on BHI agar plates. As numbers ofL. ruminus SPM 0211 were increased, viable cell count of VISA and VRE decreased. The strongest antimicrobial activity of SPM 0211 was observed after 9 h incubation in any mixture, almost completely inhibiting the growth of these two bacteria. The results suggest that the freshly isolatedL. ruminus SPM 0211 may be used as a pro-biotic microbe that prevents the colonization of enteric pathogens and can thereby promote good gastrointestinal health.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Bae, H. G., Kim, S. O., Park, J. S., Kang, B. Y., Choi, S. S., Kang, J. Y., and Ha, N. J., Analysis of Genetic Diversity ofBifidobacterium sp. Isolated from Korean Adults Fecal Samples.Yakhak Hoieji, 48(1), 20–26 (2004).
Breslaw, E. S., Kleyn D. H.,In vitro digestibility of protein in yoghurt at various stages of processing.J. Food Sci., 38, 1016 (1973).
Catherine, L. and Henry, F. C.,Staphylococcus aureus with heterogeneous resistance to vancomycin: epidemiology, clinical significance, and critical assessment of diagnostic methods.Antimicrobial Agents and Chemotherapy, 47(10), 3040–3045 (2003).
Choi, E. H., Kim, H. Y., Kim, Y. H., Kim, W. K., Oh, S. J., and Kim, Y. H., Effects of Selected Oligosaccharides on Fecal Microflora and Lipid Constitution in Rats.Korean Nutrition society J., 32(3), 221–229 (1999).
Committee on Treatment if International Union against Tuberculosis and Lung Disease: Antituberculosis regimens of chemotherapy.Bull. Int. Union Tuberc. Lung Dis., 63, 60 (1988).
David, S. J., Jasmina, D., and Sean, P. G., The resistance of polyvinylpyrrolidone-Iodine-poly(-caprolactone) blends to adherence ofEscherichia coli.Biomaterials, 26(14), 2013–2020 (2005).
Fridkin, S. K., Vancomycin-intermediate and-resistantStaphylococcus aureus: What the infectious disease specialist needs to know.Clinical Infectious Diseases, 32(1), 108–115 (2001).
Ha, K. H., Characterization of Vibrio parahaemolyticus Isolated from Diarrheal Patients and Environmental Sources by RAPD-PCR.J. Bacteriol. Virol., 34(1), 45–58 (1999).
Ha, N. J., Park, J. S., Yun, J. H., Jang, D. H., Kang, B. Y., Chung, M. J., Kim, S. D., Hong, O. P., and Choi, S. S., Genomic Fingerprinting ofBifidobacterium sp. Isolated from the Feces of Healthy Korean Adults using Microbial Microbial Uniprimer Kit.FASEB J., A992 (2004).
Kang, B. Y., Yun, J. H., Chung, M. J., Kim, S. D., Hong, O. P., Kang, J. Y., Choi, S. S., and Ha, N. J., Isolation, Identification and Characterization of potential probioticBifidobacterium sp. from Healthy Korea Adults.J. Microbiol., 42(3), (2004).
Kim, B. J., Gong, J. Y., Lee, C. S., and Lee, Y. H., Development of Remedy by for the Infectible Disease by Resistant Bacteria; Development of New Antibiotics with Antimicrobial Activity against Resistant Bacteria.Korea Research Institute Chemical Technology, 1–207 (2003).
Kim, S. H., Keon, N. H., Kim, J. Y., Lim, J. Y., Bae, W. K., Kim, J. M., Noh, K. J., Hur, J., Jung, W. K., Park, K. T., Lee, J. E., Ra, J. C., and Park, Y. H., Antimicrobial Activity of Natural Product Made by Opuntia ficus-indica var. saboten AgainstSalmonella sp. andEscherichia coli O157:H7.J. Fd. Hyg. Safety, 17(2), 71–78 (2002)
Lee, J. W., Epidemiological typing of Vancomycin-Resistant Streptococcus equines isolated from Animal Foods by Microbial Uniprimer KitTM. (2002).
Lee, K. M., Yun, J. H., Choi, S. S., and Ha, N. J., Comparison of environmental growth condition inBifidobacterium sp. parent and It's mutants.J. Life Sci., 12, 113–121 (2004).
Lee, S. H., Park, N. Y., and Choi, W. J., Changes of the Lactic Acid Bacteria and Selective Inhibitive Substances against Homo and HeteroLactic Acid bacteria Isolated from Kimchi.Kor. J. Appl. Microbiol. Biotechnol., 27(5), 410–414 (1999).
Matsuki, T., Watanabe, K., Tanaka, R., and Oyaizu, H., Rapid identification of human intestinalbifidobacteria by 16S rRNA-targeted species- and group-specific primers.FEMS Microbiology Lett., 167, 113–121 (1998)
Mcdonel, J. L., Clostridium perfringens toxins (Type A, B, C, D, E).Pharmacol. Ther., 10, 617–655 (1980).
Mitsuoka, T., Bifidobacteria and their role in human health.J. Ind. Microbiol., 6, 263–268 (1990).
Modler, H. W., Mckeller, R. C., and Yaguchi, M., Bifidobacteria and bifidogenic factors.Can. Inst. Food Sci. Technol. J., 23, 29–41 (1990).
Mullie, C., Odou, M. F., Singer, E., Romond, M. B., and Izard, D., Multiplex PCR using 16S rRNA gene-targeted primers for the identification ofbifidobacteria from human origin.FEMS Microbiology Lett., 222, 129–136 (2003).
National Committee for Clinical Labortory Standards (1988).
Olivier, D., Claire, N., Baudouin, B., Christiane, K., Sophie, B. D., and Marc, J. St., Emergence of vancomycin-intermediateStaphylococcus aureus in a Belgian hospital: microbiological and clinical features.J. Antimicrobial Chemother., 50(3), 383–391 (2002).
Park, J. C., Kim, I. S., Kwon, S. K., Noh, J. M., Lee, S. M., Park, J. P., Lee, W. K., and Ryu, S. R., Prevalence of Antibiotic-Resistant Strains among Bacteria Isolated from Bovine Mastitis, Swine Diarrhea, and Swine Pneumonia.Kor. J. Appl. Microbiol. Biotechnol., 28(4), 189–194 (2000).
Park, S. G., Park, S. K., Jung, J. H., and Jin, Y. H., Antibiotic Susceptibility of Salmonella sp. Isolated From Diarrhoea Patients in Seoul From 1996 to 2001.J. Fd. Hyg. Safety, 17(2), 61–70 (2002).
Rhee, Y. K., Han, M. J., Choi, E. C., Kim, D. H., Hypocholesterolemic activity of Bifidobacteria isolated from a healthy Korean.Arch. Pharm. Res., 25, 681 (2002).
Sekine, K., Toida, T., Saito, M., Kuboyama, M., Kawashima, T., and Hashimoto, Y., A new morphologically characterized cell wall preparation (whole peptidoglycan) fromBifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice.Cancer Res., 45, 1300 (1985).
Song, J. H., Emerging Infectious Disease due to Micorbial Adaptation: Emergence and Spread of Antimicrobial Resistance.Korean Society of Infection Disease, Infection and chemotherapy, 31(1), 79–87 (2002).
Chun, U. H., Park, B. S., and Cho, J. S., Optimum Conditions for the Protoplast Formation ofLactobacillus plantarum andLactobacillus plantarum.Korean J. Biotechnol. Bioeng., 9(2), 191–199 (1994).
Venema, K. and Maathuis, J. H., A PCR-based method for identification ofbifidobacteria from the human alimentary tract at the species level.FEMS Microbiology Lett., 224, 143–149 (2003).
Woodford, N., Johnson, A. P., Morrison, D., and Speller, D. C. E., Current perspectives on glycopeptide resistance.Clin. Microbial Rev., 8, 585–615 (1995).
Yamashita, M., Hatano, M., Matsui, T., and Kumon, Y., Effects of Bifermin-Radmistered in combination with antibiotics on the fecal flora.Bifidobacteria Microflora, 4, 23 (1985).
Yokoyama, M. T. and Carlson, J. R., Microbial metabolites of tryptophan in the intertinal tract with special reference to skatole.Am. J. Chin. Nutr., 32, 173–178 (1979).
Yu, H. J., Lee, S. S., Lee, D. S., and Kim, H. B., Isolation ofLactobacillus plantarum HB1 from Tongchimi and Its Nitrite-Scavenging Effect.Kor. J. Microbiol., 39(3), (2003).
About this article
Cite this article
Yun, JH., Yim, Ds., Kang, JY. et al. Identification ofLactobacillus ruminus SPM0211 isolated from healthy Koreans and its antimicrobial activity against some pathogens. Arch Pharm Res 28, 660–666 (2005). https://doi.org/10.1007/BF02969355
- Lactobacillus ruminus
- 16S-rRNA sequence
- Vancomycin Intermediate resistant
- Staphylococcus aureus