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High cell density cultivation ofPseudomonas oleovorans for the production of poly(3-hydroxyalkanoates)

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Abstract

Fed-batch culture ofPseudomonas oleovorans was carried out for the production of medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using octanoate as a carbon source. Octanoate and the salt solution containing ammonium sulfate and magnesium sulfate were intermittently fed in the course of fermentation. Cell mass and PHA concentrations of 42.8 and 16.8 g/L, respectively, could be obtained in 40 h. The PHA content and the PHA productivity were 39.2% and 0.42 g PHA/L-h, respectively. The yields of cell mass and PHA were 0.71 g dry cell mass/goctanoate and 0.28 g PHA/g octanoate, respectively. Therefore, octanoate can be used for the production of MCL-PHAs to a high, concentration with high productvity.

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  1. Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, 305-701, Taejon, Korea

    Sang Yup Lee

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  1. Sang Yup Lee
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Lee, S.Y. High cell density cultivation ofPseudomonas oleovorans for the production of poly(3-hydroxyalkanoates). Biotechnol. Bioprocess Eng. 1, 51–53 (1996). https://doi.org/10.1007/BF02949145

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  • DOI: https://doi.org/10.1007/BF02949145

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