Skip to main content
SpringerLink
Log in

High cell density cultivation of Escherichia coli W using sucrose as a carbon source

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Escherichia coli W was cultivated in the pH-stat fed-batch mode in a chemically defined medium containing sucrose as a sole carbon source. A final cell concentration of 105 g/L could be obtained in 36 hrs by feeding 20 g sucrose+0.57 g MgSO47H2O for a definite on-time upon the pH rise. Using the same strategy, a recombinant strain harboring pSYL104 could also be grown to 125 g/L in 48 hrs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alaeddinoglu, N.G., and Charles, H.P. (1979). J. Gen. Microbiol. 110, 47–59.

    Google Scholar 

  • Fouset, A., Klier, A., and Rapoport, G. (1982). Mol. Gen. Genet. 186, 399–404.

    Google Scholar 

  • Gerdes, K. (1988). Bio/Technol. 6, 1402–1405.

    Google Scholar 

  • Gleiser, I.E., and Bauer, S. (1981). Biotechnol. Bioeng. 23, 1015–1021.

    Google Scholar 

  • Kim, B.S., Lee, S.Y., and Chang, H.N. (1992). Biotechnol. Lett. 14, 811–816.

    Google Scholar 

  • Konstantinov, K.B., Nishio, N., and Yoshida, T. (1990). J. Ferment. Bioeng. 70, 253–260

    Google Scholar 

  • Lee, S.Y., Yim, K.S., Chang, H.N., and Chang, Y.K. (1993). J. Biotechnol., in print.

  • Riesenberg, D. (1991). Curr. Opin. Biotechnol. 2, 380–384.

    Google Scholar 

  • Riesenberg, D., Schulz, V., Knorre, W.A., Pohl, H.-D., Korz, D., Sanders, E.A., Roβ, A., and Deckwer, W.-D. (1991). J. Biotechnol. 20, 17–28.

    Google Scholar 

  • Schubert, P., Kruger, N., and Steinbuchel, A. (1991). J. Bacteriol. 173, 168–175.

    Google Scholar 

  • Steinbuchel, A. (1991). Acta Biotechnol. 11, 419–427.

    Google Scholar 

  • Suzuki, T., Yamane, T., and Shimizu, S. (1990). J. Ferment. Bioeng. 69, 292–297.

    Google Scholar 

  • Tsunekawa, H., Azuma, S., Okabe, M., Okamoto, R., and Aiba, S. (1992). Appl. Environ. Microbiol. 58, 2081–2088.

    Google Scholar 

  • Yamane, T., and Shimizu, S. (1984). Adv. Biochem. Eng. Biotechnol. 30, 147–194.

    Google Scholar 

  • Yee, L., and Blanch, H.W. (1992). Bio/Technol. 10, 1550–1556.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 373-1 Kusung-dong, 305-701, Yusung-gu, Taejon, Korea

    Sang Yup Lee & Ho Nam Chang

Authors
  1. Sang Yup Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ho Nam Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, S.Y., Chang, H.N. High cell density cultivation of Escherichia coli W using sucrose as a carbon source. Biotechnol Lett 15, 971–974 (1993). https://doi.org/10.1007/BF00131766

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00131766

Keywords

Search

Navigation