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Aerobic immobilized cells in alginate gel particles of variable density

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Abstract

Immobilized cells ofAcetobacter sp. were used as a model system of aerobic cells to study the influence of gel particle density in fermentation carried out in fluidized-bed bioreactor. Particles of variable density were prepared by adding different amounts of α-alumina to the gel matrix. The effect of the density of the particles was analyzed through the variation of their terminal velocity.

The behavior of continuous oxidation of ethanol to acetic acid was characterized in terms of productivity of acetic acid and gas-liquid volumetric oxygen transfer coefficient, as a function of dilution rate.

An empirical correlation among the gas-liquid volumetric oxygen transfer coefficient, volumetric particle concentration (εS/L), and particle Reynolds number (Re p) was proposed in this work.

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Abbreviations

C*:

saturated dissolved oxygen concentration in fermentation medium, (kmol /m3)

Cdo :

dissolved oxygen concentration in fermentation medium, (kmol/m3)

CHAc2, CHAc1 :

bioreactor outlet and inlet acetic acid concentration (g/L)

D:

dilution rate (1/h)

Kla:

gas-liquid volumetric oxygen transfer coefficient (1/h)

M:

molecular weight of acetic acid

Pr:

total acetic acid production rate (g/L·h)

Qo2t :

specific respiration rate, (kmol/kg [dry wt] h)

Rep :

particle Reynolds number (-)

vvm:

vessel volumes per minute

X:

cell concentration, (kg/m3)

εL :

liquid holdup (-)

εS/L :

volumetric particle concentration (-).

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Authors and Affiliations

  1. Biotechnological Process Development Laboratory, Chemical Engineering School, UNICAMP, C.P. 6066, CEP 13083-970, Campinas, São Paulo, Brazil

    Álvaro Alberto De Araújo & Maria Helena Andrade Santana

Authors
  1. Álvaro Alberto De Araújo
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  2. Maria Helena Andrade Santana
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De Araújo, Á.A., Santana, M.H.A. Aerobic immobilized cells in alginate gel particles of variable density. Appl Biochem Biotechnol 57, 543–550 (1996). https://doi.org/10.1007/BF02941734

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