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Adjuvant effect ofBacillus firmus in intranasal immunization of guinea pigs with inactivated type B influenza virus

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Abstract

Intranasal immunization of guinea pigs with inactivated type B influenza virus plus inactivatedBacillus firmus as an adjuvant compared to the virus alone yields higher titers of serum hemagglutination-inhibiting antibodies and virus-neutralizing antibodies. This phenomenon could be useful in standard serology, especially in the preparation of immune sera against highly pathogenic strains forin vitro diagnosis.

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Abbreviations

BF:

Bacillus firmus

CFR:

complement-fixation reaction

HIT:

hemagglutination-inhibition test

HT:

hemagglutination titer

MDCK:

Madine Darby canine kidney

TCID50 :

tissue culture-infectious dose 50 %

TW/E-HIT:

HIT with a Tween-diethyl ether split antigen

VNT:

virus-neutralization test

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Authors and Affiliations

  1. National Institute of Public Health, Centre of Epidemiology and Microbiology, 100 42, Prague, Czechia

    M. Havlíčková, J. Táčner & R. Limberková

  2. 1st Faculty of Medicine, Charles University, 120 00, Prague, Czechia

    L. Prokešová & P. Zanvit

Authors
  1. M. Havlíčková
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  2. L. Prokešová
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  3. P. Zanvit
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  4. J. Táčner
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  5. R. Limberková
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Corresponding author

Correspondence to M. Havlíčková.

Additional information

This work was supported by grants of theGrant Agency of the Czech Republic (310/02/0467/A) and theGrant Agency of Ministry of Education, Youth and Sports of the Czech Republic (MSM 0021 620 806).

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Havlíčková, M., Prokešová, L., Zanvit, P. et al. Adjuvant effect ofBacillus firmus in intranasal immunization of guinea pigs with inactivated type B influenza virus. Folia Microbiol 51, 154–156 (2006). https://doi.org/10.1007/BF02932172

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  • DOI: https://doi.org/10.1007/BF02932172

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