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Glycoproteins isolated from Atractylodes macrocephala Koidz improve protective immune response induction in a mouse model

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Abstract

This study examined the efficacy of Atractylodes macrocephala Koidz (AMK) protein and polysaccharide extracts as adjuvant or adjuvant booster when given together with porcine pleuropneumonia vaccine. Experimental mice (n = 5/group) were subcutaneously immunized with 25 μg ApxIIA #3 antigen, a target protein against A. pleuropneumoniae, together with alum and/or various concentrations (0–500 μg) of the AMK extracts, while the control group received PBS only. Immunization with ApxIIA #3 antigen increased the antigen-specific IgG titer and this increase was enhanced in the immunization together with AMK protein, but not polysaccharide extract. Supplementation of AMK protein extract exhibited dose-dependent increases in the antigen-induced protective immunity against A. pleuropneumoniae challenge and in the lymphocyte proliferation specific to the antigen. Glycoproteins present in the AMK extract were the active components responsible for immune response induction. Collectively, the present findings suggest that AMK glycoproteins are useful as immune stimulating adjuvant or adjuvant booster.

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Acknowledgements

This work was also supported by a grant from the RDA, Ministry of Agriculture and Forestry, Republic of Korea (Grant No. PJ013394022018).

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Authors and Affiliations

  1. Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea

    Kyoung-A. Kim, Young-Hyun Baek, Chun-Chu Kim, Jeong-Hoon Lee & Jeong-Chae Lee

  2. Cell Dynamics Research Center and School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, South Korea

    Young-Ok Son

  3. Department of Bioactive Material Sciences, Research Center of Bioactive Materials and Institute of Oral Bioscience, Chonbuk National University, Jeonju, 54896, South Korea

    So-Soon Kim, Yong-Suk Jang & Jeong-Chae Lee

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  1. Kyoung-A. Kim
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Correspondence to Jeong-Chae Lee.

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This research proposal was approved by the Chonbuk National University Committee on Ethics in the Care and Use of Laboratory Animals, which is considered as our institutional animal care and use committee in scientific research.

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Kim, KA., Son, YO., Kim, SS. et al. Glycoproteins isolated from Atractylodes macrocephala Koidz improve protective immune response induction in a mouse model. Food Sci Biotechnol 27, 1823–1831 (2018). https://doi.org/10.1007/s10068-018-0430-1

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  • DOI: https://doi.org/10.1007/s10068-018-0430-1

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