Abstract
Functions of T cells were determined after intranasal and intratracheal immunization of mice with ovalbumin (Ova) andBacillus firmus (Bf), a Gram-positive nonpathogenic bacterium of the external environment, or delipidatedBf (dBf) as adjuvants, with the aim to elucidate the mechanism of support of Ova-specific antibody production caused byBf that had been observed in an identical experiment. NeitherBf nor dBf in a mixture with Ova stimulated Ova-specific T-cell response tested as antigen-specific blast transformation. By contrast, a mild polyclonal stimulation was observed in splenocytes from mice given dBf. In vitro incubation of splenocytes with 100 µg (but not 10 µg) ofBf or dBf led to a highly significant inhibition of proliferation below the control level in all groups of animals. Supernatants of splenocyte cultures were further tested for cytokine production. IL-10 and IFN-γ were released afterin vitro challenge with dBf and in some cases also withBf. Analysis of sera demonstrated that administration of Ova + adjuvant brought about an increase in anti-Ova IgG1, IgG2a and IgG2b whereas treatment with Ova alone caused a rise in IgG1 only. The role ofBf or dBf in the enhancement of antigen-specific antibody production could be in influencing macrophages and inducing cytokine milieu composed of IL-10, IFN-γ and other factors that leads to a bystander stimulation of specifically activated Ova-B cell receptor (Ova-BCR)-bearing cells.
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Abbreviations
- Bf :
-
Bacillus firmus
- BCR:
-
B-cell receptor
- ConA:
-
concanavalin A
- CT:
-
cholera toxin
- dBf :
-
delipidatedBacillus firmus
- FCS:
-
fetal calf serum
- i.n.:
-
intranasal
- i.t.:
-
intratracheal
- LPS:
-
lipopolysacharide
- LT:
-
heat-labile toxin ofE. coli
- Ova:
-
ovalbumin
- PBS:
-
phosphate-buffered saline
- PRR:
-
pattern recognition receptors
- PAMP:
-
pathogen-associated molecular patterns
- ROI:
-
reactive oxygen intermediates
- RNI:
-
reactive nitrogen intermediates
- RTDC:
-
respiratory tract dendritic cells
- SI:
-
stimulation indices
- TCR:
-
T-cell receptor
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This work was supported by grants VS 96149. MSM 111 100 005 and FRVS 1895/2000 of theMinistry of Education, Youth and Sports of the Czech Republic.
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Mlčková, P., Čechová, D., Marušková, L. et al. Role of T cells in the adjuvant effect ofbacillus firmus on the immune system of mice: Intranasal and intratracheal immunization study with ovalbumin. Folia Microbiol 48, 427–434 (2003). https://doi.org/10.1007/BF02931379
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DOI: https://doi.org/10.1007/BF02931379