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Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator

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Abstract

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination ofin situ removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis membrane and equipped on the agitator of spinner reactor forin situ removal of ammonium ion. The semi-fed-batch operation was employed to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

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Authors and Affiliations

  1. Division of Food Science and Biotechnology, Kangwon National University, 200-701, Chunchon, Korea

    Byong-Gon Park, Joo-Mi Chun, Chang-Jin Lee & Yeon-Ho Jeong

  2. Division of Life Sciences, Kangwon National University, 200-701, Chunchon, Korea

    Gie-Taek Chun

  3. Graduate School of Biotechnology, Korea University, 136-701, Seoul, Korea

    Ik-Hwan Kim

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  1. Byong-Gon Park
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  2. Joo-Mi Chun
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  4. Gie-Taek Chun
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  6. Yeon-Ho Jeong
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Correspondence to Yeon-Ho Jeong.

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Park, BG., Chun, JM., Lee, CJ. et al. Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator. Biotechnol. Bioprocess Eng. 5, 123–129 (2000). https://doi.org/10.1007/BF02931883

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  • DOI: https://doi.org/10.1007/BF02931883

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