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ClinicalPseudomonas aeruginosa: Potential factors of pathogenicity and resistance to antimicrobials

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Abstract

Resistance to 17 antimicrobials, surface hydrophobicity, motility, biofilm, production ofN-acylhomoserine lactone signal molecules (N-butyrylhomoserine lactone andN-3-oxolauroylhomoserine lactone) and response to oxidative stress were analyzed in 47 clinicalPseudomonas aeruginosa strains. In addition to natural resistance, the strains demonstrated the greatest level of resistance to cefotaxime (91.5 %). Isolates in the range of 44.7–57.4 % were resistant to aminoglycosides and ciprofloxacin, of 25.5–36.2 % to cephalosporins. On the other hand, 97.9 % remained susceptible to meropenem, 93.6 % to piperacillin + tazobactam and 87.2% to piperacillin. The majority of the strains (72.3 %) manifested their hydrophilic character. Higher zones of motility showed 12 isolates (in average 54.8 mm) as compared to the others (30.2 mm). Approximately 1/3 of the strains (29.8 %) produced a higher amount of biofilm quantified by measuring the absorbance of solubilized crystal violet (0.20–0.46) than the rest of isolates (0–0.19). All but two strains producedN-3-oxolauroylhomoserine lactone and in 48.9 % of samplesN-butyrylhomoserine lactone were detected. Only four isolates with higher biofilm production showed both types of homoserine lactone. Majority of the strains (70.2 %) manifested higher resistance to H2O2 than the rest of the strains. The group of strains resistant to aminoglycosides and ciprofloxacin revealed a significantly higher number of hydrophobic strains (compared with the sensitive ones). In contrast, higher number of strains sensitive to aminoglycosides and ciprofloxacin or only to ciprofloxacin producedN-butyrylhomoserine lactone and biofilm (compared to the resistant ones). Such association was not found among the rest of the tested parameters. The results indicate that the resistance to antimicrobials inP. aeruginosa isolates was not generally associated with changes in the production of the pathogenicity factors.

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Abbreviations

HSL:

homoserine lactone(s)

3-oxo-C12-HSL:

N-3-oxolauroylhomoserine lactone

C4-HSL:

N-butyrylhomoserine lactone

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Authors and Affiliations

  1. Slovak Medical University, 833 03, Bratislava, Slovakia

    A. Hoštacká & I. Čižnár

  2. Institute of Microbiology, Medical Faculty and Faculty Hospital, Comenius University, Bratislava, Slovakia

    L. Slobodníková & D. Kotulová

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  1. A. Hoštacká
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  2. I. Čižnár
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  3. L. Slobodníková
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  4. D. Kotulová
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Hoštacká, A., Čižnár, I., Slobodníková, L. et al. ClinicalPseudomonas aeruginosa: Potential factors of pathogenicity and resistance to antimicrobials. Folia Microbiol 51, 633–638 (2006). https://doi.org/10.1007/BF02931631

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