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Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defense mechanisms

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Abstract

To investigate whether hemocytes ofBombyx mori (Lepidoptera) larvae produce reactive oxygen species (ROS) as part of the oxidative killing of invading pathogens, the production of ROS was measured as a luminol- and lucigenin-enhanced chemiluminescence of unstimulated or stimulated (zymosan particles, phorbol myristate acetate, calcium ionophore, rice starch orXenorhabdus nematophila) hemolymph. No detectable ROS production was found. The spontaneous and activated ROS production measured with hemocytes,i.e. under the conditions when the antioxidative potential of hemolymph plasma was eliminated, was again undetectable. Likewise, ROS production by isolated hemocytes was observed by spectrophotometric (NBT test, cytochromec assay) and fluorimetric (using dihydrorhodamine and hydroethidine probes) methods. Hence none of the experimental approaches used indicated the production of ROS by hemocytes ofB. mori larvae as part of their immune response.

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Authors and Affiliations

  1. Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, 611 37, Brno, Czechia

    P. Hyršl

  2. Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65, Brno, Czechia

    M. Číž, L. Kubala & A. Lojek

Authors
  1. P. Hyršl
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  2. M. Číž
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  3. L. Kubala
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  4. A. Lojek
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Corresponding author

Correspondence to A. Lojek.

Additional information

This study was conducted as part of the research project Z 500 4920 and was supported by grant no. S 500 4009 of theGrant Agency of the Academy of Sciences of the Czech Republic.

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Hyršl, P., Číž, M., Kubala, L. et al. Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defense mechanisms. Folia Microbiol 49, 315–319 (2004). https://doi.org/10.1007/BF02931049

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  • DOI: https://doi.org/10.1007/BF02931049

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