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Isolation of xylose reductase gene ofPichia stipitis and its expression inSaccharomyces cerevisiae

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Abstract

A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast,Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced inSaccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation inP stipitis were inducible.S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol byS. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.

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Authors and Affiliations

  1. International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamada- oka, Suita-shi, Osaka, 565, Japan

    Shinya Takuma, Noriyuki Nakashima, Manee Tantirungkij, Shinichi Kinoshita, Hirosuke Okada, TatsÜji Sew & Toshiomi Yoshida

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  1. Shinya Takuma
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  2. Noriyuki Nakashima
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  3. Manee Tantirungkij
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  4. Shinichi Kinoshita
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  5. Hirosuke Okada
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  6. TatsÜji Sew
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  7. Toshiomi Yoshida
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Takuma, S., Nakashima, N., Tantirungkij, M. et al. Isolation of xylose reductase gene ofPichia stipitis and its expression inSaccharomyces cerevisiae . Appl Biochem Biotechnol 28, 327–340 (1991). https://doi.org/10.1007/BF02922612

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