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The Impact of Transcriptional Factors Znf1 and Sip4 on Xylose Alcoholic Fermentation in Recombinant Strains of Yeast Saccharomyces Cerevisiae

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Abstract—In recent years, the demand for technical ethanol has increased due to its use in the transport sector. Xylose is the major five-carbon sugar obtained as a result of lignocellulose hydrolysis; however, the industrial producer of alcohol S. cerevisiae yeast ferments exclusively hexoses. Based on a recombinant strain capable of xylose metabolism, the derivatives with increased expression of the ZNF1 gene and deletion of the SIP4 gene encoding transcription factors were constructed. It was found that overexpression of the ZNF1 gene did not affect the fermentation of glucose or xylose. The deletion of the SIP4 gene did not affect the fermentation of glucose but resulted in a 29% decrease in ethanol production during xylose fermentation in comparison with the parental strain.

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Funding

The study was partially supported by the grant of the Polish National Science Center (NCN) DEC- 2012/05/B/ NZ1/01657.

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Authors and Affiliations

  1. Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005, Lviv, Ukraine

    L. S. Dzanaeva, A. A. Sibirny & K. V. Dmytruk

  2. University of Rzeszow, 35-601, Rzeszow, Poland

    J. Ruchala & A. A. Sibirny

Authors
  1. L. S. Dzanaeva
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  2. J. Ruchala
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  3. A. A. Sibirny
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  4. K. V. Dmytruk
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Correspondence to K. V. Dmytruk.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by V. Mittova

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Dzanaeva, L.S., Ruchala, J., Sibirny, A.A. et al. The Impact of Transcriptional Factors Znf1 and Sip4 on Xylose Alcoholic Fermentation in Recombinant Strains of Yeast Saccharomyces Cerevisiae . Cytol. Genet. 54, 386–392 (2020). https://doi.org/10.3103/S0095452720050035

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  • DOI: https://doi.org/10.3103/S0095452720050035

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