Abstract
Bacillus stearothermophilus BR219, a phenol-resistant thermophile, can convert phenol to the specialty chemical catechol. The growth kinetics of this organism were studied in batch, continuous, and im-mobilized-cell culture. Batch growth was insensitive to pH between 6.0 and 8.0, but little growth occurred at 5.5. In continuous culture on a dilute medium supplemented with 10 mM phenol, several steady states were achieved between dilution rates of 0.25 and 1.3 h-1. Phenol degradation was found to be uncoupled from growth. Immobilized cells grew rapidly in a rich medium, but cell viability plummeted following a switch to a dilute medium supplemented with 5 mM phenol.
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Worden, R.M., Subramanian, R., Bly, M.J. et al. growth kinetics ofbacillus stearothermophilus BR219. Appl Biochem Biotechnol 28, 267–275 (1991). https://doi.org/10.1007/BF02922606
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DOI: https://doi.org/10.1007/BF02922606