Abstract
Mortierella alpina ATCC 32222 grew well at 11°C, as well as at 25°C in a liquid medium containing glucose or linseed oil and yeast extract. High Eicosapentaenoic acid (EPA) yield was obtained at 11°C.M. alpina cells did not produce EPA at 25°C in the absence of linseed oil, whereas at 11°C, EPA accumulation was noted in the absence of linseed oil. When grown at 11°C for 10 d in a medium containing 2% linseed oil as carbon source, the mycelium yielded 435 mg/L EPA (20 mg EPA/g dry mycelia) with 5.1% in lipid fraction. By gradually increasing the concentration of linseed oil to 4%, yield of biomass and EPA were increased to 43 g/L and 596 mg/L, respectively.
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Bajpai, P., Bajpai, P.K. & Ward, O.P. Eicosapentaenoic acid (EPA) Production byMortierella alpina ATCC 32222. Appl Biochem Biotechnol 31, 267–272 (1991). https://doi.org/10.1007/BF02921753
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DOI: https://doi.org/10.1007/BF02921753