Abstract
The preparation of antisera against S alleles of homozygous incompatible lines of the marrow-stem kale (Brassica oleracea var.acephala DC.) is described. The antisera obtained reacted specifically with homologous antigens on using the serological method of double diffusion into agar. The results confirmed the specificity of S alleles of incompatible lines of the marrow-stem kale.
Abstract
Příspěvek popisuje přípravu antisér proti S alelám inkompatibilních linií krmné kapusty. Získaná antisera reagovala specificky s homologními antigeny při použití sérologické metody dvojí difuze do agaru. Výsledky potvrdily specificitu S alel homozygotních inkompatibilních linií krmné kapusty.
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References
Haruta, T.: Studies of the genetics of self-and cross-incompatibility in cruciferous vegetables.— Takii Plant Breeding and Exp. Sta. Kyoto, Jap. Res. Bull., 2, 1962.
Kakizaki, J.: Studies on the genetics and physiology of self-and cross-incompatibility in the common cabbage.—Jap. J. Bot.5: 134–208, 1930.
Lewis, D.: Serological reactions of pollen incompatibility substances.—Proc. roy. Soc. (London)B 140: 127–135, 1952.
Nasrallah, M. E., Barber, J. T., Wallace, D. H.: Self-incompatibility proteins in plants: detection, genetics, and possible mode of action.—Heredity25: 23–27, 1970
Nasrallah, M. E., Wallace, D. H.: Immunochemical detection of incompatibility antigens in stigmas of self-incompatible and self-fertile inbreds ofBrassica oleracea var.capitata.—Geneties54: 351, 1966.
Nasrallah, M. E., Wallace, D. H.: Immunogenetics of self-incompatibility inBrassica oleracea L.—Heredity22: 519–527, 1967.
Ouchterlony, O.: Antigen-antibody reactions in gels.—Acta pathol. microbiol. scand.26: 507 to 515, 1949.
Pearson, O. H.: Breeding plants of the cabbage group.—Calif. agr. Exp. Sta. Bull. 532, 1932.
Thompson, K. F.: Classified S alleles forBrassica breeders.—In: Proc. of Brassica Meeting of Eucarpia, Hort. section, 1968.
Thompson, K. F., Howard, H. W.: Self-incompatibility in marrow-stem kale,Brassica oleracea var.acephala. II. Methods for the recognition in inbred lines of plants homozygous for S alleles. —J. Genet.56: 325–340, 1959.
Wallace, D. H., Nasrallah, M. E.: Pollination and serological procedures for isolating incompatibility genotypes in the crucifers.—Mem. Cornell Univ. agr. Exp. Sta.: 406–423, 1968.
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Kučera, V., Polák, J. The serological specificity of S alleles of homozygous incompatible lines of the marrow-stem kale (Brassica oleracea var.acephala DC.). Biol Plant 17, 50–54 (1975). https://doi.org/10.1007/BF02921074
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DOI: https://doi.org/10.1007/BF02921074