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Viele monoklonale Antikörper sind nicht monospezifisch

  • Hybridome
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Abstract

Sequencing of antibody mRNA of 185 randomly selected hybridoma clones revealed that an alarming third of them produces additional antibody chains. In case such chains are expressed, a single hybridoma cell secretes a mixture of IgG with different specificities. ELISA and immunohistology confirmed the negative effect of such additional chains on specificity and apparent affinity. Therefore, many monoclonal antibody preparations can be not monospecific.

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Authors and Affiliations

  1. Institut für Biochemie, Biotechnologie und Bioinformatik, TU Braunschweig, Braunschweig, Deutschland

    Stefan Dübel

  2. Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Spielmannstraße 7, D-38106, Braunschweig, Deutschland

    Stefan Dübel

Authors
  1. Stefan Dübel
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Correspondence to Stefan Dübel.

Additional information

Stefan Dübel Jahrgang 1960. 1978–1984 Biologiestudium an den Universitäten Mainz und Heidelberg. 1989 Promotion an der Universität Heidelberg. 1989–1995 Postdoc am Deutschen Krebsforschungszentrum, Heidelberg. 1995–2001 Arbeitsgruppenleiter am Institut für Molekulare Genetik, Universität Heidelberg. Seit 2002 Lehrstuhl für Biotechnologie an der TU Braunschweig.

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Dübel, S. Viele monoklonale Antikörper sind nicht monospezifisch. Biospektrum 24, 395–399 (2018). https://doi.org/10.1007/s12268-018-0935-4

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  • DOI: https://doi.org/10.1007/s12268-018-0935-4

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