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The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein)

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Abstract

Was studied in vitro. The109Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd2+ from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place.

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Authors and Affiliations

  1. Department of Veterinary Pathology, Faculty of Veterinary Medicine, State University, PO Box 80.158, 3508 TD, Utrecht, The Netherlands

    H. Meulenaar & T. S. G. A. M. van den Ingh

  2. Interuniversity Reactor Institute, Delft, The Netherlands

    C. J. A. van den Hamer & T. M. W. Stolk

Authors
  1. H. Meulenaar
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  2. C. J. A. van den Hamer
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  3. T. S. G. A. M. van den Ingh
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  4. T. M. W. Stolk
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Meulenaar, H., van den Hamer, C.J.A., van den Ingh, T.S.G.A.M. et al. The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein). Biol Trace Elem Res 7, 49–54 (1985). https://doi.org/10.1007/BF02916546

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  • DOI: https://doi.org/10.1007/BF02916546

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