Abstract
The pRi T-DNA markers were followed in the first generation of the progeny ofAgrobacterium tumefaciens strain C58Cl(pRiA4b) transformed tobacco regenerant. Two categories segregating probably in the ratio 15: 1 can be distinguished: those showing a complex of pRi T-DNA transformation markers and those showing no transformation markers. The presence of both TL and TR-DNA was demonstrated by Southern blotting. The most prominent of the transformation markers observedIn vitro was rapid growth of detached plagiotropic roots on media without phytohormones. Most of the progeny plants of the transformant showed irregularities of leaf morphology and inflorescence and flower morphology. The last resulted in sterility in most progeny plants.
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OndRej, M., Hrouda, M., KoneCnÁ, A. et al. Progeny study of tobacco regenerant transformed by agrobacterium rhizogenes. Biol Plant 31, 280–285 (1989). https://doi.org/10.1007/BF02907289
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DOI: https://doi.org/10.1007/BF02907289