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Analysis of sister chromatid exchange and chromosome replication kinetics using BrdU-dye techniques

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Virchows Archiv B

Summary

The basis of BrdU-dye techniques for detecting DNA synthesis in cytological preparations is reviewed. General procedural modifications are described for applying this approach for the study of chromosome replication kinetics and sister chromatid exchange formation. Implications of sister chromatid exchange analysis for detecting mutagen-carcinogens and for differentiating human chromosome fragility diseases predisposing to neoplasia are discussed.

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Authors and Affiliations

  1. Center for Human Genetics and Department of Pediatrics, Harvard Medical School, USA

    Samuel A. Latt

  2. Mental Retardation Center, Children’s Hospital Medical Center, Boston, Massachusetts

    Samuel A. Latt

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  1. Samuel A. Latt
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Latt, S.A. Analysis of sister chromatid exchange and chromosome replication kinetics using BrdU-dye techniques. Virchows Arch. B Cell Path. 29, 19–27 (1978). https://doi.org/10.1007/BF02899332

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  • DOI: https://doi.org/10.1007/BF02899332

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