Abstract
Sister chromatid exchanges are sharply demarcated in Giemsa stained metaphase preparations of Chinese hamster ovary cells and human peripheral leukocytes. Chromatids singly and doubly substituted with BrdU acquire differential Giemsa stain affinities after treatment at 88° C for 10 minutes in 1.0 M Na phosphate buffer (pH 8.0).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bridges, C. B.: Non-disjunction as proof of the chromosome theory of heredity. Genetics 1, 1–52, 107–163 (1916)
Bridges, C. B., Anderson, E. G.: Crossing over in the X chromosomes of triploid females of Drosophila melanogaster. Genetics 10, 418–441 (1925)
Chu, E. H. Y., Sun, N. C., Chang, C. C.: Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light. Proc. nat. Acad. Sci. (Wash.) 69, 3459–3463 (1972)
Creighton, H. B., McClintock, B.: A correlation of cytological and genetical crossing-over in Zea mays. Proc. nat. Acad. Sci. (Wash.) 17, 492–497 (1931)
Freese, E.: The specific mutagenic effect of base analogues on phage T4. J. molec. Biol. 1, 87–105 (1959)
Ham, R. G.: An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res. 29, 515–526 (1963)
Hsu, T. C., Somers, C. E.: Effect of 5-bromodeoxyuridine on mammalian chromosomes. Proc. nat. Acad. Sci. (Wash.) 47, 396–403 (1961)
Kato, K., Moriwaki, K.: Factors involved in the production of banded structures in mammalian chromosomes. Chromosoma (Berl.) 38, 105–120 (1972)
Latt, S.A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Latt, S. A.: Localization of sister chromatid exchanges in human chromosomes. Science 185, 74–76 (1974)
McClintock, B.: The production of homozygous deficient tissues with mutant characteristics by means of the aberrant mitotic behavior of ring-shaped chromosomes. Genetics 23, 315–376 (1938)
McClintock, B.: Spontaneous alterations in chromosome size and form in Zea mays. Cold Spr. Harb. Symp. quant. Biol. 9, 72–81 (1941)
Peacock, W. J.: Chromosome duplication and structure as determined by autoradiography. Proc. nat. Acad. Sci. (Wash.) 49, 793–801 (1963)
Sehested, J.: A simple method for R banding of human chromosomes, showing a pH-dependent connection between R and G bands. Humangenetik 21, 55–58 (1974)
Stern, C.: Zytologisch-genetische Untersuchungen als Beweise für die Morgansche Theorie des Faktorenaustauschs. Biol. Zbl. 51, 547–587 (1931)
Taylor, J. H.: Sister chromatid exchanges in tritium-labeled chromosomes. Genetics 43, 515–529 (1958)
Taylor, J. H., Woods, P. S., Hughes, W. L.: The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labelled thymidine. Proc. nat. Acad. Sci. (Wash.) 43, 122–128 (1957)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Korenberg, J.R., Freedlender, E.F. Giemsa technique for the detection of sister chromatid exchanges. Chromosoma 48, 355–360 (1974). https://doi.org/10.1007/BF00290992
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00290992