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Bromodeoxyuridine tablet methodology for in vivo

Studies of DNA synthesis

  • Published:
Somatic Cell Genetics

Abstract

5-Bromodeoxyuridine (BrdU) tablets with different physical characteristics are useful in a wide variety of studies requiring detection of DNA replication in vivo. These tablets can effect a high substitution of BrdU in DNA, thereby permitting sister chromatid differentiation in chromosomes stained with 33258 Hoechst alone or in conjunction with Giemsa. Baseline and cyclophosphamide-induced in vivo sister chromatid exchange frequencies in mouse spleen, marrow, and thymus were measured and found to be significantly greater than those in spermatogonia. Sister chromatid exchange analysis was also extended to mouse liver and to Chinese hamster and Armenian hamster marrow cells. Sister chromatid differentiation was observed in Armenian hamster meiotic tissue, and evidence for interhomolog chromatid exchange obtained.

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Authors and Affiliations

  1. Genetics Division and Mental Retardation Center, Children's Hospital Medical Center, the Department of Pediatrics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts

    James W. Allen, Charles F. Shuler & Samuel A. Latt

  2. National Institute of Environmental Health Sciences, Research Triangle, North Carolina

    James W. Allen

Authors
  1. James W. Allen
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  2. Charles F. Shuler
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  3. Samuel A. Latt
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Allen, J.W., Shuler, C.F. & Latt, S.A. Bromodeoxyuridine tablet methodology for in vivo. Somat Cell Mol Genet 4, 393–405 (1978). https://doi.org/10.1007/BF01538862

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  • DOI: https://doi.org/10.1007/BF01538862

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