Skip to main content
Log in

In vitro propagation of oak (Quereus robur L.) and linden (Tilia cordata Mill.)

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Rapid multiplication of axillary shoots of oak and linden has been achieved on broad-leaved tree medium (BTM) and woody plant medium (WPM) containing low level of cytokinin (BAP 0.2–1.0 mg l-1). High rooting percentages (80–95%) were obtained on low salt, low sucrose media, containing low level of auxins. Rooted plants were transplanted into pots containing a mixture of peat and perlite. Most of the plants (90–95%) survived the transfer. After the hardening off period the new plants were planted in the field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Brown, C. L., Sommer, H. E.: Vegetative propagation of dicotyledonous trees. - In:Bonga, J.M., Durzan, D. J. (ed.): Tissue Culture in Forestry. Pp. 109–149. Martinus Nijhoff/Dr. W. Jung Publishers, The Hague-Boston-London 1982.

    Google Scholar 

  • Chalupa, V.:In vitro propagation of some broad-leaved forest trees. - Commun. Inst. Forest. Čechosl.11: 159–170, 1979.

    Google Scholar 

  • Chalupa, V.: Clonal propagation of broad-leaved forest treesin vitro. - Commun. Inst. Forest. Cechosl.12: 253–271, 1981.

    Google Scholar 

  • Gresshoff, P. M., Doy, C. H.: Development and differentiation of haploidLycopersicon esculentum, (tomato). -Planta107: 161–170, 1972.

    Article  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Smith, M. A. L., McCown, B. H.: A comparison of source tissue for protoplast isolation from three woody plant species. - Plant Sci. Lett.28: 149–156, 1983.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chalupa, V. In vitro propagation of oak (Quereus robur L.) and linden (Tilia cordata Mill.). Biol Plant 26, 374–377 (1984). https://doi.org/10.1007/BF02898577

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02898577

Keywords

Navigation