Skip to main content
SpringerLink
Log in

Development and differentiation of haploid Lycopersicon esculentum (tomato)

  • Published:
Planta Aims and scope Submit manuscript

Summary

Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture. This is a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana. Differentiation of haploid callus of Lycopersicon esculentum can be controlled both in the dark and the light by hormones added to defined minimal media. Development to plantlets is achieved only in the light. Callus cells can be induced to develop into seedless pseudo-fruits. Chromosome counts on callus cells or root-tip cells establishes haploidy (n=12).

Haploidy can be maintained in culture on defined minimal media for at least one year.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Blaydes, D. F.: Interaction of kinetic and various inhibitors in the growth of soy bean tissue. Physiol. Plantar. 19, 748–753 (1966).

    Google Scholar 

  • Darlington, C. D., LaCour, L. F.: Handling of chromosomes, 2nd edn., revised. London: George Allen and Unwin 1947.

    Google Scholar 

  • Doy, C. H., Gresshoff, P. M., Rolfe, B.: Phage-mediated transfer and subsequent expression of the galactose operon from Escherichia coli in cells of haploid tomato callus. Proc. Austr. Biochem. Soc. 5 (1972a).

  • Doy, C. H., Gresshoff, P. M., Rolfe, B.: Transgenosis of bacterial genes from Escherichia coli to cultured cells of haploid Lycopersicon esculentum. Proc. Int. Meeting “The biochemistry of gene expression in higher organisms”. (1972b).

  • Gamborg, O. L., Eveleigh, D.: Culture methods and detection of glucanases in suspension cultures of wheat and barley. Canad. J. Biochem. 46, 417–421 (1968).

    Google Scholar 

  • Gresshoff, P. M., Doy, C. H.: Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. biol. Sci. 25, 259–264 (1972).

    Google Scholar 

  • Guha, S., Maheshwari, S. C.: Cell division and differentiation of embryos in pollen grains of Datura in vitro. Nature (Lond.) 212, 97–98 (1966).

    Google Scholar 

  • Nataka, K., Tanaka, M.: Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jap. J. Genet. 43, 65–71 (1968).

    Google Scholar 

  • Sunderland, N., Wicks, F. M.: Cultivation of haploid plants from tobacco pollen. Nature (Lond.) 244, 1227–1229 (1969).

    Google Scholar 

  • Sunderland, N., Wicks, F. M.: Embryoid formation in pollen grains of Nicotiana tabacum. J. exp. Bot. 22, 213–221 (1971).

    Google Scholar 

  • Sunderland, N.: Anther culture: a progress report. Sci. Progr. Oxf. 59, 527–549 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra City, A. C. T., Australia

    Peter M. Gresshoff & Colin H. Doy

Authors
  1. Peter M. Gresshoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Colin H. Doy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gresshoff, P.M., Doy, C.H. Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107, 161–170 (1972). https://doi.org/10.1007/BF00387721

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00387721

Keywords

Search

Navigation