Summary
Increased numbers of nuclear bodies are observed in littorial cells as well as in fatty infiltrated parenchymal cells of rat liver 6 hrs after 3/4-hepateetomy. In the parenchymals cells most of these nuclear bodies contain fat droplets. No forms are found which suggest a prolapse of fat droplets into the nucleus by invaginations of the cytoplasm with or without loss of the nuclear membrane, as occurs in cases of long term liver injury. It is believed that fat enteres the nucleus without visibly deforming on the nuclear membrane and induces the formation of the fat-containing nuclear bodies. The same mechanism pertains for glycogen- and protein-containing nuclear bodies. All these forms are interpreted to be functional structures in which substances primarly derivedfrom outside the nucleus are separated from the karyoplasm. Other nuclear bodies contain different forms of granules, i.e., “ribosome-like” granules and interchromatinic granules. These granules apparently represent a material derivedfrom the nucleus itself and separated within nuclear bodies as the above-mentioned substances.
Zusammenfassung
6 Std nach 3/4-Hepatektomie werden sowohl in den stets verfetteten Leberepithelien wie in den Wandzellen der Sinusoide auffallend zahlreiche Karyosphäridien (nuclear bodies) gefunden. In den Epithelien enthalten sie in der Regel tropfiges Neutralfett. Diese Form des intranukleären Fettes ist von derjenigen nach Plasmainvagination (mit und ohne sekundären Membranverlust) abzugrenzen. Es wird angenommen, daß die Fettsubstanzen der Karyosphäridien dem Cytoplasma entstammen, ohne gröbere Durchbrechung der Kernhülle in das Kerninnere gelangt sind und hier die Bildung von nuclear-bodies induziert haben. Der gleiche Entstehungsmechanismus wird auch für glycogen- und proteinhaltige Sphäridien vertreten. All diese Formen werden als ad hoc gebildete karyoplasmatische Funktionsstrukturen gedeutet, dazu bestimmt, in kleinen Portionen eingedrungeneskernfremdes Material intranucleär auszusondern. Für die granulären Formen der Karyosphäridien, die entweder ribosomenartige Partikel oder Interchromatingranula enthalten, wird eine ähnliche Aussonderungsfunktion erwogen, nur mit dem Unterschied, daß sie hierkerneigenes Material betrifft.
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Altmann, H.W., Pfeifer, U. Beitrag zur Kenntnis der Karyosphäridien („nuclear-bodies“). Fetthaltige Sphäridien nach partieller Hepatektomie. Virchows Arch. Abt. B Zellpath. 2, 220–228 (1969). https://doi.org/10.1007/BF02889585
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DOI: https://doi.org/10.1007/BF02889585