Abstract
A new “cryobiopsy” (CB) technique has been invented and applied for mice livers in vivo without stopping their blood circulation. They were routinely freeze-substituted in acetone containing fixatives for light microscopy (LM) or freeze-fractured before freeze substitution for scanning electron microscopy (SEM). Serial paraffin sections were stained with hematoxylin-eosin (HE) or histochemical periodic acid-Schiff (PAS) reaction. By HE-staining, the tissue surface areas were often compressed and sinusoidal erythrocytes got aggregated side by side. But in a little deeper tissue areas, hepatic sinusoids were widely open with flowing erythrocytes. Lots of PAS-reaction products were well preserved in hepatocytes of the CB specimens. To the contrary, they were unevenly distributed in hepatocytes of conventionally quick-frozen specimens or often lost in those of conventional dehydrated specimens. By SEM, some cell organelles, open Disse’s spaces, and bile canaliculi appeared under normal blood circulation samples. The new CB technique would be useful for examining time-dependent morphological changes of functioning organs, including the livers, from an identical living animal.
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Fujii, Y., Ohno, N., Terada, N., Ohno, S. (2016). Morphological and Histochemical Analysis of Living Mouse Livers by New “Cryobiopsy” Technique. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_47
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DOI: https://doi.org/10.1007/978-4-431-55723-4_47
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