Summary
The effect of estrogen on cell proliferation in the descending colon of the mouse as an example of a non-target organ was investigated. Ovariectomized mice were given single or multiple injections of 10 ng/g body weight of 17β-estradiol and were killed 1 h after3H-thymidine injection. Estrogen treatments decreased incorporation of3H-thymidine into the DNA of colonic mucosa most markedly at 4 h after the single or the last of multiple injections. The inhibitory effect of estrogen on3H-thymidine incorporation was greater and lasted longer after a single injection than after multiple ones. A similar inhibitory effect was observed in the colonie mucosa of male mice as well as in the mucosa of mice in which colonie epithelial cell proliferation was enhanced by refeeding after 48 h of fasting. However, the colonie mucosa of mice treated with estrogen implants for up to 4 days was not affected. Estrogen treatments caused no significant change in the DNA, RNA and protein contents of the colonie mucosa. The efficacy of estrogen treatments was verified by an increase in both the wet and dry weights of the uterine horns of ovariectomized mice.
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References
Bullough WS (1946) Mitotic activity in the adult female mouse, Mus musculus L. A study of its relation to the oestrous cycle in normal and abnormal conditions. Phil Trans R Soc London Series B 231:453–516
Burton K (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323
Chang WWL (1971) Renewal of the epithelium in the descending colon of the mouse. III. Diurnal variation in the proliferative activity of epithelial cells. Am J Anat 131:111–119
Crafts RC (1941) The effect of endocrines on the formed elements of the blood. II. The effect of estrogens in the dog and monkey. Endocrinology 29:606–618
Dukes PP, Goldwasser E (1961) Inhibition of erythropoiesis by estrogen. Endocrinology 69:21–29
Epifanova OI (1966) Mitotic cycles in estrogen-treated mice: a radioautographic study. Exp Cell Res 42:562–577
Epifanova OI (1971) Effects of hormones on the cell cycle. In: Baserga R (ed) The cell cycle and cancer. Marcel Dekker, Inc., New York, N.Y., pp 145–190
Frankfurt OS (1968) Effect of hydrocortisone, adrenalin and actimomycin D on transition of cells to the DNA synthesis phase. Exp Cell Res 52:220–232
Galand P, Rodesch F, Leroy F, Chretien J (1967) Altered duration of DNA synthesis and cell cycle in non-target tissues of mice treated with oestrogen. Nature 216:1211–1212
Gorski J, Noteboom WD, Nicolette JA (1965) Estrogen control of the synthesis of RNA and protein in the uterus. J Cell Comp Physiol 66 (Suppl 1):91–109
Hamilton TH (1968) Control by estrogen of genetic transcription and translation. Science 161:649–661
Hoff MB, Chang WWL (1979) The effect of estrogen on epithelial cell proliferation and differentiation in the crypt of the descending colon of the mouse. A radioautographic study. Am J Anat 155:507–516
Lee AE (1972) Cell division and DNA synthesis in the mouse uterus during continuous oestrogen treatment. J Endocrinol 55:507–513
Lee AE, Rogers LA (1972) Cell division in the mouse vagina during continuous oestrogen stimulation. J Endocrinol 54:357–358
Legan SJ, Coon GA, Karsch FJ (1975) Role of estrogen as initiator of daily LH surges in the ovariectomized rat. Endocrinology 96:50–56
Lin RIS, Schjeide OA (1969) Micro-estimation of RNA by the cupric ion catalayzed orcinol reaction. Analyt Biochem 27:473–483
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–276
Martin L, Finn CA, Trinder G (1973) Hypertrophy and hyperplasia in the mouse uterus after oestrogen treatment: an autoradiographic study. J Endocrinol 56:133–144
Schmidt G, Thannhauser SJ (1945) A method for the determination of deoxyribonucleic acid, ribonucleic acid phosphoproteins in animal tissues. J Biol Chem 161:83–89
Stormshak F, Leake R, Wertz N, Gorski J (1976) Stimulatory and inhibitory effects of estrogen on uterine DNA synthesis. Endocrinology 99:1501–1511
Szego CM, Roberts S (1953) Steroid action and interaction in uterine metabolism. Rec Progr Horm Res 8:419–469
Vollmer EP, Gordon AS (1941) Effect of sex and gonadotropic hormones upon the blood picture of the rat. Endocrinology 29:828–837
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Hoff, M.B., Chang, W.W.L. & Mak, K.M. Effect of estrogen on cell proliferation in colonic mucosa of the mouse. Virchows Archiv B Cell Pathol 35, 263–273 (1980). https://doi.org/10.1007/BF02889166
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DOI: https://doi.org/10.1007/BF02889166