Summary
The incorporation of3H-proline into the hyaline articular cartilage of rats with and without adjuvant-induced arthritis was investigated 1 h after the intravenous injection of radioactive proline. In the control animals, silver grains were present predominantly over the chondrocytes at all times; on the 28th day the intensity of labelling was notably reduced. In the animals with adjuvant disease reduced radioactivity was observed in the cartilage of the arthritic joints from the 14th experimental day onwards. At all times, however, silver grains occurred over the chondrocytes, including those in the pannus-covered cartilage and in cartilage sequesters. It is concluded from the investigations that the arthritic process accompanying the development of pannus reduces collagen synthesis and that the pannus tissue ingrows vital cartilage.
Zusammenfassung
Die Inkorporation von3H-Prolin in den hyalinen Gelenkknorpel von Ratten mit und ohne Adjuvansarthritis wurde 1 h nach der intravenösen Injektion des radioaktiven Prolins untersucht. Bei den Kontrolltieren fanden sich Silberkörner zu allen Zeitpunkten vorwiegend über den Chondrocyten, am 28. Tage war die Markierung ausgesprochen gering. Bei den Versuchstieren war in dem Knorpel der arthritischen Gelenke vom 14. Versuchstage an weniger radioaktiv markiertes Material vorhanden. Zu allen Zeitpunkten kamen aber auch unter dem pannusbedeckten Knorpel und in Knorpelsequestern Silberkörner über den Chondrocyten vor. Aus den Untersuchungen wird geschlossen, daß der arthritische Prozeß, der mit einer Pannusentwicklung einhergeht, die Kollagensynthese reduziert und daß das Pannusgewebe in einen vitalen Knorpel einwächst.
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The investigations were supported by a grant from the Deutsche Forschungsgemeinschaft (Mo 183/5)
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Mohr, W., Wild, A. & Wolf, H.P. Incorporation of3H-proline into hyaline articular cartilage. Virchows Arch. B Cell Path. 28, 1–12 (1978). https://doi.org/10.1007/BF02889051
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DOI: https://doi.org/10.1007/BF02889051