Summary
Alteration of cytosolic free calcium ([Ca2+ 1]) in hypoxia was studied with fluorescent probe, Fura-2 in cultured porcine pulmonary arterial endothelial cells. It was found that hypoxia caused by bubbling the cell suspension with 100% N2 increased [Ca2+ 2] in endothelial cells by 90±20% (n=8,P<0.05), but didn’t affect that in a Ca2+-free buffer, La3+ (2×10−5 mol/L) inhibited the hypoxia-induced increment in [Ca2+]1, whereas verapamil (10−8 mol/L) didn’t. These findings suggest that hypoxia could induce Ca2+ influx in pulmonary arterial endochelial cells, which might play an important role in vascular response to hypoxia.
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References
Von Euler US, et al. Observation on the pulmonary artery blood pressure in the cat. Acta physiol Scan, 1946,12:301
Rubangi GM, et al. Hypoxia releases a vasoconstrictor substance from the canine vascular endothelium. J Physiol, 1985,364:45
Holded WE, et al. Hypoxic-induced contraction of porcine pulmonary artery strips depends on intact endothelium. Exp Lung Res, 1984,7:101
Rodman DM, et al. Hypoxia contraction of isolated rat pulmonary artery. J Pharmacol Exp Therapeutics, 1989, 248:952
Mathew R, et al. O2 and pulmonary artery tone: effects of endothelium, Ca2+, cyanide, and monocrotaline. J, Appl Physiol, 1991,71(1):30
Adams DJ, et al. Ion channels and regulation of intracellular calcium in vascular endothelial cells. FAEBS J, 1989,3:2389
Margaret CS, et al. Bradykinin-induced increases in cytosolic calcium and ionic currents in cultured bovine aortic endothelial cells. Circulation Research, 1987,61:632
Salvaterra CG, et al. Direct effect of hypoxia on apparent intracellular calcium levels in cultured pulmonary vascular smooth muscle cells. Am Rev Res Dis, 1991, 143:A373
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Qing-hua, H., Di-xun, W. Hypoxia increases cytosolic free calcium in porcine pulmonary arterial endothelial cells. Journal of Tongji Medical University 13, 14–17 (1993). https://doi.org/10.1007/BF02886586
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DOI: https://doi.org/10.1007/BF02886586