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Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

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TRP Channels in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

Hypoxic pulmonary vasoconstriction (HPV) is an essential physiological mechanism of the lung which matches perfusion to ventilation to optimize gas exchange. Pulmonary arterial smooth muscle cells (PASMC) are the effector and possibly also the sensor cells of HPV. Contraction of these cells under hypoxia is induced by an increase in intracellular Ca2+ concentration ([Ca2+]i). To investigate details of the hypoxia-induced increase in [Ca2+]i, we use a live cell imaging procedure with fura-2 in isolated wild-type (WT) and gene-deficient mouse PASMC. We also describe here the manganese-quenching method to determine the role of nonselective cation influx through transient receptor potential (TRP) channels in response to hypoxia.

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Author information

Authors and Affiliations

  1. Excellence Cluster Cardio-Pulmonary System, University of Giessen Lung Center, Department of Internal Medicine II, Justus-Liebig-University Giessen, Giessen, Germany

    Beate Fuchs & Norbert Weissmann

  2. Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA

    Hermann Kalwa

  3. Walther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University Munich, Munich, Germany

    Thomas Gudermann & Alexander Dietrich

Authors
  1. Beate Fuchs
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  2. Hermann Kalwa
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  3. Norbert Weissmann
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  4. Thomas Gudermann
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  5. Alexander Dietrich
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Corresponding author

Correspondence to Beate Fuchs .

Editor information

Editors and Affiliations

  1. , Transfusion Services, Monmouth Medical Center, Second Avenue 300, Long Branch, 07740, New Jersey, USA

    Arpad Szallasi

  2. Medical and Health Science Center, Department of Physiology, University of Debrecen, Nagyerdei Krt. 98, Debrecen, 4032, Hungary

    Tamás Bíró

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Fuchs, B., Kalwa, H., Weissmann, N., Gudermann, T., Dietrich, A. (2012). Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells. In: Szallasi, A., Bíró, T. (eds) TRP Channels in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-077-9_15

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  • DOI: https://doi.org/10.1007/978-1-62703-077-9_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-076-2

  • Online ISBN: 978-1-62703-077-9

  • eBook Packages: Springer Protocols

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