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Effect of hypoxia on TRPV1 and TRPV4 channels in rat pulmonary arterial smooth muscle cells

  • Ion channels, receptors and transporters
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Abstract

Transient receptor potential (TRP) channels of the vanilloid subfamily, mainly TRPV1 and TRPV4, are expressed in pulmonary artery smooth muscle cells (PASMC) and implicated in the remodeling of pulmonary artery, a landmark of pulmonary hypertension (PH). Among a variety of PH subtypes, PH of group 3 are mostly related to a prolonged hypoxia exposure occurring in a variety of chronic lung diseases. In the present study, we thus investigated the role of hypoxia on TRPV1 and TRPV4 channels independently of the increased pulmonary arterial pressure that occurs during PH. We isolated PASMC from normoxic rat and cultured these cells under in vitro hypoxia. Using microspectrofluorimetry and the patch-clamp technique, we showed that hypoxia (1 % O2 for 48 h) significantly increased stretch- and TRPV4-induced calcium responses. qRT-PCR, Western blotting, and immunostaining experiments revealed that the expression of TRPV1 and TRPV4 was not enhanced under hypoxic conditions, but we observed a membrane translocation of TRPV1. Furthermore, hypoxia induced a reorganization of the F-actin cytoskeleton, the tubulin, and intermediate filament networks (immunostaining experiments), associated with an enhanced TRPV1- and TRPV4-induced migratory response (wound-healing assay). Finally, as assessed by immunostaining, exposure to in vitro hypoxia elicited a significant increase in NFATc4 nuclear localization. Cyclosporin A and BAPTA-AM inhibited NFATc4 translocation, indicating the activation of the Ca2+/calcineurin/NFAT pathway. In conclusion, these data point out the effect of hypoxia on TRPV1 and TRPV4 channels in rat PASMC, suggesting that these channels can act as direct signal transducers in the pathophysiology of PH.

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Acknowledgments

The authors thank Dr. Arnaud Courtois for his helpful discussions. This work was supported by the “Fonds de Dotation pour la Recherche en Santé Respiratoire” (grant 2012/2013).

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    Authors and Affiliations

    1. Univ. Bordeaux, Centre de Recherche Cardio-Thoracique de Bordeaux, U1045, F-33000, Bordeaux, France

      Thibaud Parpaite, Guillaume Cardouat, Marthe Mauroux, Jennifer Gillibert-Duplantier, Paul Robillard, Jean-François Quignard, Roger Marthan, Jean-Pierre Savineau & Thomas Ducret

    2. INSERM, Centre de Recherche Cardio-Thoracique de Bordeaux, U1045, F-33000, Bordeaux, France

      Thibaud Parpaite, Guillaume Cardouat, Marthe Mauroux, Jennifer Gillibert-Duplantier, Paul Robillard, Jean-François Quignard, Roger Marthan, Jean-Pierre Savineau & Thomas Ducret

    3. Centre de Recherche Cardio-Thoracique de Bordeaux, INSERM U1045, Université de Bordeaux, 146 rue Léo-Saignat (case 13), F-33076, Bordeaux cedex, France

      Thomas Ducret

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    1. Thibaud Parpaite
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    Correspondence to Thomas Ducret.

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    Guillaume Cardouat and Marthe Mauroux contributed equally to this work.

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    Parpaite, T., Cardouat, G., Mauroux, M. et al. Effect of hypoxia on TRPV1 and TRPV4 channels in rat pulmonary arterial smooth muscle cells. Pflugers Arch - Eur J Physiol 468, 111–130 (2016). https://doi.org/10.1007/s00424-015-1704-6

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