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Molecular source of biomarkers by genetic engineering techniques

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Chinese Science Bulletin

Abstract

The mutant lacking ORF469 fragment inSynechocystis sp. PCC 6803 (cyanobacterium) was created by means of DNA recombination. In its genome,ORF 469, the key DNA fragment controlling the light-independent pathway of chlorophyll biosynthesis was deleted and replaced by erythromycin resistance cassette. The operation resulted in the fact that the content of chlorophyll in mutant cells was fully controlled by illumination and two kinds of cells were harvested, one is high chlorophyll with concentration of 9.427 μg· mg−1 and the other is low chlorophyll with concentration of 0.695 μg · mg1. They were subjected to thermal simulation respectively at 300°C for 100 h. The alkanes biomarkers from pyrolysates were analyzed by GC-MS and main difference between high and low chlorophyll cells was found at their contents of isoprenoid hydrocarbons. Pr/nC17 and Ph/nC18 from pyrolysate of low chlorophyll cells were 0.192 and 0.216 respectively, which were about 1/3 and 1/7 of that from high chlorophyll cells. The results provide direct evidence that isoprenoid hydrocarbons such as phytane(Ph) and pristane (Pr) could be derived from chlorophyll. The lipids in algal cells would be the most important contributors to hydrocarbon production in their thermal degradation. The results also indicated that the combination of molecular biology and organic geochemistry would provide a new path to investigate the molecular sources of biomarkers.

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References

  1. Peters, K. E., Moldowan, J. M., The biomarker guider: interpreting molecular fossils in petroleum and ancient sediments, Englewood Cliffs, New Jersery: Prentice Hall, Inc. A Simon & Schuster Company, 1993, 110, 233.

    Google Scholar 

  2. Fu Jiamo, Sheng Guoying, Advances of molecular organic geochemistry in China, Progress in Natural Science, 1995, 5(3): 270.

    CAS  Google Scholar 

  3. Wu Qingyu. Yin Shi, Shen Guoyin et al., Distribution and comparison of steranes from blue-green algae under different simulated conditions, Science in China, Ser. B, 1993, 36(7): 872.

    CAS  Google Scholar 

  4. Wu Qingyu, Yin Shi, Sheng Guoyin et al, New discoveries in study on liquid hydrocarbons from thermal degradation of heterotrophically yellowing algae. Science in China, Ser. B, 1994, 37: 326.

    CAS  Google Scholar 

  5. Anja Miltner, Wolfgang Zech., Effect of minerals on the transformation of organic matter during simulated fire-induced pyrolysis, Org. Geochem., 1997, 26(3/4): 175.

    Google Scholar 

  6. Geoffrey, D., Abbott, B. B. et al., The thermal degradation of 5α(H)-cholestane during closed-system pyrolysis, Geochim. Cosmochim. Acta, 1995. 59(11): 2259.

    Article  Google Scholar 

  7. Domine, F., High pressure pyrolysis of n-hexane, 2,4-dimethylpentane and 1-phenylbutane, Is pressure an important geochemical parameter? Org. Geochem., 1991, 17(5): 619.

    Article  CAS  Google Scholar 

  8. Villanueva, J., Grimait, J. O., de Wit, R. et al., Sources and transformations of chlorophylls and carotenoids in a monomictic sulphate-rich karstic lake environment, Org. Geochem., 1994, 22(6): 739.

    Article  CAS  Google Scholar 

  9. Yuko Soma, Atsushi Tanaka, Mitsuyuki Sonia et al., Photosynthesis pigments and perylene in the sediments of southern basin of Lake Baikal, Org. Geochem., 1996, 24(5): 553.

    Article  CAS  Google Scholar 

  10. Wu Qingyu, Vermaas, W. F. J., Molecular cloning and deleting-mutant construction of ORF469 inSynechocystis sp. PCC 6803, Chinese Journal of Biotechnology, 1996, 12(3): 311.

    Google Scholar 

  11. Anderson, S. L., Mcintosh, L., Light-activated heterotrophic growth of the cyanobacteriumSynechocystis sp. PCC 6803: a blue-light-requiting process,. J. Bacteriol., 1991, 173:2761.

    PubMed  CAS  Google Scholar 

  12. Wang Ruiyong, Zhou Wen, Dai Junbiao et al., Identification of long chain isoprenoid hydrocarbon in the pyrolyzed productof Dunaliella, Chinese Science Bulletin, 1999, 44(11): 1190.

    Google Scholar 

  13. Wu Qingyu, Xu Hong, Biosynthesis of chlorophyll regulates reconstruction of thylakiod menbrane in cyanobacteriumSynechocystis sp. PCC 6803, Acta Botanic Sinica, 1997, 39(11): 1003.

    Google Scholar 

  14. Keely, B. J., Prowse, W. G., Maxwell, J. R., The treibs hypothesis: An evaluation based on structural studies, Energy & Fuel, 1990, 4:628.

    Article  CAS  Google Scholar 

  15. Ishiwatari, M., Ishiwalari, R., Sakashila H. et al., The effect of preheating on the pyrolysis of chlorophyll-α: simulation of diagenetic process in kerogen formation, Chem. Lett., 1990, 875.

  16. Ishiwatari, M., Ishiwatari, R., Yamada, K., Two possible pathways of formation of pristane and phytane from chlorophylls on diagenesis: carbon isotopic results from laboratory heating of chlorophyll-a, Chem. Lett., 1997, 355.

  17. John, M. G., Molecular and carbon isotopic analysis of individual biological markers: evidence for sources of organic matter and paleoenviunmental conditions in the Upper Ordovician Maquoketa Group, Illinois Basin, U.S.A. Org. Geochem., 1996, 25(8): 439.

    Google Scholar 

  18. Wu Qingyu, Zhang Bin, Song Yitao et al., Effects of hydrolysis and bacterial degradation in the cells on hydrocarbons and biomarkers generated by pyrolysis of green alga Chlorella Protothecoides, Chinese Science Bulletin, 1998, 43(9): 749.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, China

    Junbiao Dai, Qingyu Wu, Ruiyong Wang & Weimin Peng

  2. Institute of Guangzhou Geochemistry, Chinese Academy Sciences, 510640, Guangzhou, China

    Guoyin Shen & Jiamo Fu

Authors
  1. Junbiao Dai
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  2. Qingyu Wu
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  3. Ruiyong Wang
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  4. Weimin Peng
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  5. Guoyin Shen
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  6. Jiamo Fu
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Corresponding author

Correspondence to Qingyu Wu.

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Cite this article

Dai, J., Wu, Q., Wang, R. et al. Molecular source of biomarkers by genetic engineering techniques. Chin. Sci. Bull. 45, 1025–1030 (2000). https://doi.org/10.1007/BF02884985

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  • DOI: https://doi.org/10.1007/BF02884985

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