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Comparison of reputed duplicate populations in the Russian and US Potato Genebanks using RAPD Markers

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Abstract

The Association of Potato Intergenebank Collaborators (APIC) produced a global inventory of wild potato genetic resources that is available on the Internet (www.potgenebank.org/ipd). This database shows that, in many cases, several genebanks have samples of the progeny from a single original germplasm collection. The assumption has been that these samples are genetically equivalent, so all the characterization and evaluation data gathered on a seedlot from one genebank can be applied to all the other “duplicate” seedlots in other genebanks. This assumption was tested by comparing 25 pairs of reputed duplicates in the VIE (St. Petersburg, Russia) and US (Sturgeon Bay, USA) potato genebanks using RAPDs. In 23 of 25 populations, reputed duplicates among genebanks had significantly less similarity than replicate samples taken from a single population. The average genetic similarity of reputed duplicates was 93%, and the lowest was 81%. Thus, users of germplasm should be aware that reputed duplicate populations from these genebanks may not be genetically identical.

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Abbreviations

VIR:

Vavilov Institute Potato Genebank

GS:

Genetic similarity

RAPD:

Random Amplified Polymorphic DNA

Literature Cited

  • Bamberg, J., C. Singsit, A.H. del Rio, and E.B. Radcliffe. 1999. RAPD analysis of genetic diversity in Solanum populations to predict the need for fine screening. Am J Potato Res 77:275–278.

    Article  Google Scholar 

  • del Rio, A.H., and J.B. Bamberg. 1998. Effects of sampling size and RAPD marker heterogeneity on the estimation of genetic relationships. Am J Potato Res 75:275.

    Google Scholar 

  • del Rio, A.H., J.B. Bamberg, and Z. Huaman. 1997. Assessing changes in the genetic diversity of potato genebanks. 1. Effects of seed increase. Theor Appl Genet 95:191–198.

    Article  Google Scholar 

  • Divaret, I., E. Margale, and G. Thomas. 1999. RAPD markers on seed bulks efficiently assess the genetic diversity of aBrassica oleraceae L. collection. Theor Appl Genet 98:1029–1035.

    Article  CAS  Google Scholar 

  • Gilbert, J.E., R.V. Lewis, M.J. Wilkinson, and P.D.S. Caligary. 1999. Developing an appropriate strategy to assess genetic variability in plants germplasm collections. Theor Appl Genet 98:1125–1131.

    Article  CAS  Google Scholar 

  • Hanneman Jr., R.E. 1989. The potato germplasm resource. Am Potato J 66:665–667.

    Article  Google Scholar 

  • Hosaka, K., and R.E. Hanneman Jr. 1991. Seed protein variation within populations of wild and cultivated potato species and inbredSolanum ckacoense. Potato Res 34:419–428.

    Article  Google Scholar 

  • Huaman, Z., R. Hoekstra, and J. B. Bamberg. 2000. The Inter-genebank Potato Database and the dimensions of available wild potato germplasm. Am J Potato Res 77:353–362.

    Google Scholar 

  • Kiru, S.D., and V.P. Sdvizhkova 1999. Katalog mirovoi collectzü VIR N 707 - Kartofel - Culturny vidSolanum andigenum Juz. et Buk. Vavilov Institute (VIR, St. Petersburg, Russia) publication No. 707.22 pp.

    Google Scholar 

  • Steiner, A.M., P. Ruckenbauer, and E. Goecke. 1997. Maintenance in genebanks, a case study: Contaminations observed in the Nürnberg oats of 1831. Genet Resources Crop Evol 44:533–538.

    Article  Google Scholar 

  • Tinker N.A., D.E. Mather, and M.G. Fortin. 1994. Pooled DNA for linkage analysis: practical and statistical considerations. Genome 37:999–1004.

    CAS  PubMed  Google Scholar 

  • Williams, C.E., and P.C. Ronald. 1994. PCR template-DNA isolated quickly from monocot and dicot leaves without tissue homoge-nization. Nucleic Acids Res 22:1917–1918.

    Article  PubMed  CAS  Google Scholar 

  • Williams, G.K., R.S. Reiter, R.M. Young, and P.A. Scolnick. 1993. Genetic mapping of mutations using phenotypic tools and mapped RAPD markers. Nucleic Acids Res 21:2697–2702.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. USDA/Agricultural Research Service, Vegetable Crops Research Unit, Inter-Regional Potato Introduction Station, 4312 Hwy. 42, 54235, Sturgeon Bay, WI

    J. B. Bamberg

  2. N. I. Vavilov Institute Potato Genebank, 42 Hertzen Street, 190000, St. Petersburg, Russia

    S. D. Kiru

  3. Dept. of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706, Madison, WI

    A. H. del Rio

Authors
  1. J. B. Bamberg
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  2. S. D. Kiru
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  3. A. H. del Rio
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Bamberg, J.B., Kiru, S.D. & del Rio, A.H. Comparison of reputed duplicate populations in the Russian and US Potato Genebanks using RAPD Markers. Am. J. Pot Res 78, 365–369 (2001). https://doi.org/10.1007/BF02884345

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  • DOI: https://doi.org/10.1007/BF02884345

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