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Comparison of growth rates of two clones ofBacillus subtilis, producer ofα-amylase

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Abstract

In a population of the productiveBacillus subtilis strain the production of α-amylase differentiates at the cell level. Individual cells in the population were analyzed and the production of isolates was tested. The mean specific activity of α-amylase in the productive group after the isolation was 5.0 U/mg dry substance and in the nonproductive group 1.5 U/mg dry substance. This ratio remained unchanged during long-term observations and after repeated transfers. In selected strains of both groups the specific growth rate was determined in a synthetic medium containing various amounts of casein hydrolyzate. The detected differences in the growth rate between the productive and the nonproductive clones are determined by amino acid concentration.

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Authors and Affiliations

  1. Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    J. Pazlarová & Z. Fencl

  2. Institute of Microbiology, Soviet Academy of Sciences, Moscow

    L. A. Yegorova & L. G. Loginova

Authors
  1. J. Pazlarová
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  2. L. A. Yegorova
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  3. Z. Fencl
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  4. L. G. Loginova
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Pazlarová, J., Yegorova, L.A., Fencl, Z. et al. Comparison of growth rates of two clones ofBacillus subtilis, producer ofα-amylase. Folia Microbiol 27, 323–327 (1982). https://doi.org/10.1007/BF02883133

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  • DOI: https://doi.org/10.1007/BF02883133

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