Abstract
In order to improve the biosynthesis of amylolytic enzymes by industrial Aspergillus strains, the efficiency of stepwise application of the following methods of induced mutagenesis was studied: ultraviolet (UV) irradiation, gamma irradiation, and treatment with N-methyl-N-nitro-N-nitrosoguanidine (NG). It was found that at the early stages of mutagenesis of the glucoamylase-producing A. awamori strain UV and NG, used either alone or in combination, were efficient enough as mutagenic agents, providing an increase of glucoamylase activity by 30–35 and 50–60%, respectively. At the later stages, serial UV mutagenesis and gamma-irradiation showed high efficiency for both A. awamori, and A. oryzae strains. Gamma-mutagenesis of Aspergillus strains using a cobalt source provided the most stable and highly active strains retaining 90–95% of their activity after five transfers on agar medium. The experiments resulted in significant improvement of the studied industrial strains, more than doubling activity of the target enzymes.
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Original Russian Text © E.V. Kostyleva, A.S. Sereda, I.A. Velikoretskaya, E.I. Burtseva, T.N. Veselkina, L.I. Nefedova, A.Yu. Sharikov, N.V. Tsurikova, N.S. Lobanov, A.P. Sinitsyn, 2017, published in Mikrobiologiya, 2017, Vol. 86, No. 4, pp. 483–493.
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Kostyleva, E.V., Sereda, A.S., Velikoretskaya, I.A. et al. Development of schemes of induced mutagenesis for improving the productivity of Aspergillus strains producing amylolytic enzymes. Microbiology 86, 493–502 (2017). https://doi.org/10.1134/S0026261717040087
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DOI: https://doi.org/10.1134/S0026261717040087