Summary
B. subtilis A 18, a producer of exocellular amylase, was found to carry covalently closed DNA plasmid molecules (pMI 10). The pMI 10 was isolated and characterized by electron microscopy, electrophoretic mobility and restriction endonuclease cleavage pattern. The pMI 10 was absent in all α-amylase low productive or nonproductive clones. The pMI 10 DNA was transformed together with pUB 110 DNA into B. subtilis RM 125 arg-leu- recipient cells, and, hence, compatibility of these plasmids could be demonstrated.
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Communicated by H. Böhme
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Tichý, P., Pazlarová, J., Hartmann, M. et al. Isolation of the pMI 10 plasmid from the α-amylase producing strain of Bacillus subtilis . Molec. Gen. Genet. 181, 248–253 (1981). https://doi.org/10.1007/BF00268433
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DOI: https://doi.org/10.1007/BF00268433