Skip to main content
SpringerLink
Log in

Separation of transport and metabolic steps in the uptake of succinate byKluyveromyces fragilis

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Characteristics of succinate uptake were determined in glucose-, glycerol-, and succinate-grown cells of the succinate-utilizingKluyveromyces fragilis. Glucose represses the uptake when present during growth and inhibits it when present during uptake. Absence of glucose or presence of succinate or glycerol derepresses the uptake. The transport and subsequent metabolic steps can be efficiently separated by inhibiting the latter with carboxin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baranowski K., Radler F.: The glucose-dependent transport of L-malate inZygosaccharomyces bailii.Antonie van Leeuwenhoek50, 329–340 (1984).

    Article  PubMed  CAS  Google Scholar 

  • Read S.M., Northcate D.H.: Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein.Anal.Biochem.116, 53–04 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Rottenberg H.: The measurement of membrane potential and pH in cells, organelles, and vesicles.Methods Enzymol.55 F, 547–569 (1979).

    Article  Google Scholar 

  • Singer T.P., Massey V., Kearney E.B.: Studies on succinic dehydrogenase. V. Isolation and properties of the dehydrogenase from baker’s yeast.Arch.Biochem.Biophys.69, 405–421 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Singer T.P., Kearney E.B.: Determination of succinic dehydrogenase activity, pp. 307–333 inMethods in Biochemical Analysis (D. Glick. ed.), vol. 4. Interscience, New York 1957.

    Chapter  Google Scholar 

  • Ulrich J.T., Mathré D.E.: Mode of action of oxanthiin systemic fungicides. V. Effect on electron transport system ofUstilngo maydis andSaccharomyces cerevisiae.J.Bacteriol.110, 628–632 (1972).

    PubMed  CAS  Google Scholar 

  • White G.A.: A potent effect of 1,4-oxanthiin systemic fungicides on succinate oxidation by a particulate preparation fromUstilago maydis.Biochem.Biophys.Res.Commun.44, 1212–1219 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Wolfinbarger L., Kay W.W.: Transport of C4-dicarboxylic acids inNeurospora crassa.Biochim. Biophys.Acta307, 243–257 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Zmijewski M.J., Mao Quillan A.M.: Dual effects of glucose on dicarboxylic acid transport inKluyveromyces lactis.Can.J.Microbiol.21, 473–480 (1975).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    M. Opekarová & K. Sigler

Authors
  1. M. Opekarová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Sigler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Opekarová, M., Sigler, K. Separation of transport and metabolic steps in the uptake of succinate byKluyveromyces fragilis . Folia Microbiol 32, 200–205 (1987). https://doi.org/10.1007/BF02881100

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02881100

Keywords

Search

Navigation