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Activation of Alternative Respiration with Internal Electron Acceptor during Anaerobic Glucose Utilization in Escherichia coli Strains with Impaired Fermentation Ability

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Abstract

The activation of alternative respiration with an internal electron acceptor during anaerobic glucose utilization in E. coli strains with impaired fermentation ability has been studied. It was found out that respiration processes utilizing pyruvic acid as an endogenous electron acceptor can markedly contribute to the maintenance of the anaerobic redox balance in E. coli strains deficient in mixed acid fermentation pathways. The sequential inactivation of the pathways of anaerobic dissimilation of pyruvate and impairment of the functionality of the reductive branch of the tricarboxylic acid cycle led to an increase in the contribution (from 11 to 54%) of the respiratory formation of lactic acid and alanine to the biosynthesis of the reduced products of anaerobic glucose utilization by the strains. Analysis of the enantiomeric composition of the lactic acid and alanine secreted by the strains demonstrated that D-lactate dehydrogenase (Dld), L-lactate dehydrogenase (LctD), and D-alanine dehydrogenase (DadA) participated in the biosynthesis of the respective compounds.

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Funding

The work was supported by a grant from the Russian Foundation for Basic Research (project no. 18-04-01222).

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Authors and Affiliations

  1. State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Resource Center (GosNIIgenetika, Kurchatov Institute NRC), 117545, Moscow, Russia

    A. Yu. Skorokhodova, A. V. Sukhozhenko, A. Yu. Gulevich & V. G. Debabov

Authors
  1. A. Yu. Skorokhodova
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  2. A. V. Sukhozhenko
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  3. A. Yu. Gulevich
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  4. V. G. Debabov
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Correspondence to A. Yu. Skorokhodova.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by I. Gordon

Abbreviations: cAMP, cyclic adenosine monophosphate; CL—culture liquid; Cm—chloramphenicol; CRP—cAMP receptor protein; GS—glyoxylate shunt; HPLC—high-performance liquid chromatography; NADH—nicotinamide adenine dinucleotide reduced; OAA—oxaloacetic acid; PCR—polymerase chain reaction; PEP—phosphoenolpyruvate; TCA cycle—tricarboxylic acid cycle.

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Skorokhodova, A.Y., Sukhozhenko, A.V., Gulevich, A.Y. et al. Activation of Alternative Respiration with Internal Electron Acceptor during Anaerobic Glucose Utilization in Escherichia coli Strains with Impaired Fermentation Ability. Appl Biochem Microbiol 55, 870–876 (2019). https://doi.org/10.1134/S0003683819090072

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