Abstract
Lethal and mutagenic effects ofN-nitroso-N-ethylurea (NEU) on the parasitic fungusCercospora beticola Sacc. was investigated. Mutation frequency increased and the number of surviving individuals (conidia) simultaneously decreased with increasing mutagen concentration and the period of its application.
Treatment ofC. beticola conidia with NEU induced 14 morphological mutants characterized by changes in pigmentation of air mycelium and substrate, excretion of the pigment into the cultivation medium and colonies morphology. A considerable proportion of morphological mutants lost their sporulation ability bothin vitro on the sporulation medium andin vivo on host leaves and became non-pathogenic. The other morphological mutants (21.4%) were pathogenic on a sensitive sugar beet cultivar Dobrovická A and on other three resistant cultivars (Maribo 1, 2, 3).
A revertant with increased pathogenity arose from the light non-pathogenic mutant. During the remonosporic isolation of the pathogenic mutants isolates were obtained characterized by a phenotype which originated during the mutagenic process.
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References
Abbondandolo, A., Simi, S.: Mosaicism and lethal sectoring in G1 cells ofSchizosaccharomyces pombae. -Mut. Res.12: 143–150, 1971.
Bajaj, B. S., Chatrath, M. S., Vasudeva, R. S.: Mutation inColletotrichum falcatum Went. the causal organism of rod sugarcancer. IV. Morphological and biochemical mutants induced by ionizing radiation. - Indian Phytopathol.4: 296–303, 1964.
Boone, D. M., Keitt, G. W.:Venturia inaequalis (Cke) Wint. VIII. Inheritance of color mutant characters. -Amer. J. Bot.43: 226–233, 1956.
Boone, D. M., Stauffer, J. F., Stahmann, M. A., Keitt, G. W.:Venturia inaequalis (Cke) Wint. VII. Induction of mutants for studies on genetics, nutrition, and pathogenicity. - Amer. J. Bot.43: 199–204, 1956.
Campbell, R., Larner, R. W., Madelin, M. F.: Notes on an albino mutant ofAlternaria brassicola. - Mycologia60: 1122–1125, 1968.
Canova, A.: [Research on Biology and Epidemiology ofCercospora beticola Sacc.] In Ital. - Agraria, Roma 1959.
Dutta, S. K., Garber, E. D.: Genetics of phytopathogenie fungi. III. An attempt to demonstrate the parasexual cycle inColletotrichum lagenarium. - Bot. Gaz.122: 118–121, 1960.
Eckardt, F., Haynes, R. H.: Induction of pure and sectored mutant clones in excision-proficient and deficient strains of yeast. - Mut, Res.43: 327–338, 1977.
Frandsen, N. O.: Untersuchungen überCercospora beticola III. Morphologie dos Pilzes. - Zucker8: 513–515, 1955.
Guglielminetti, R.: The role of lethal sectoring in the origin of complete mutations inSchizosaccharomyces pombae. - Mut. Res.5: 225–229, 1968.
Hannan, M. A., Duck, P., Nasim, A.: UV-induced lethal sectoring and pure mutant clones in yeast. -Mut. Res.36: 171–176, 1976.
Hastie, A. C.: Genetic recombination in the hop-wilt fungusVerticillium albo-atrum. - J. gen. Microbiol.27: 373–382, 1962.
James, A. P., Nasim, A., McCullough, R. S.: The nature of replicating instability in yeast. -Mut. Res.15: 125–133, 1972.
James, A. P., Werner, M. M.: Radiation induced genetic mosaicism and lethal sectoring in yeast. - Mut. Res.3: 477–485, 1966.
Keitt, G. W., Boone, D. M.: Induction and inheritance of mutant characters inVenturia inaequalis in relation to its pathogenicity. - Phytopathology44: 362–370, 1954.
Levitin, M. M., Zakharov, I. A.: [The lethal and mutagenie effect of UV rays in the cause of browning and stem break of flax.] In Russ. - Genetika9: 65 - 70, 1967.
Nasim, A.: The induction of replicating instabilities by mutagens inSchizosaccharomyces pombae. - Mut. Res.4: 753–763, 1967.
Nasim, A., Grant, C.: Genetic analysis of replicating instabilities in yeast. - Mut. Res.17: 185 to 190, 1973.
Novotel’nova, N. S., Pystina, K. A.: [Induced variability of the fungus causing the verticillium wilt of cotton plants.] In Russ. - Mikol. Fitopatol.4: 290–300, 1968.
Plotho, O.: Weitere Untersuchungen zur Zytologie und Morphologie derCercospora beticola. - Zucker5: 379–383, 1952.
Remneva, Z. I., Ivanyuk, V. G.: [Interspecific inhomogeneity inMacrosporium solani Ell. et Mart. the cause of early blight of potato.] In Russ. - Mikol. Fitopatol.3: 202–208, 1968.
Tinline, R. D., Stauffer, J. F., Dickson, J. G.:Cochlyobolus sativus. III. Effect of ultraviolet radiation. - Can. J. Bot.38: 275–282, 1960.
Tuveson, R. W., Garber, E. D.: Genetics of phytopathogenic fungi. II. The parasexual cycle inFusarium oxysporum f. pisi. - Bot. Gaz.121: 74–80, 1959.
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Brilová, D. The effect ofN-nitroso-N-ethylurea on mutagenesis ofCercospora beticola SACC. Biol Plant 24, 415–422 (1982). https://doi.org/10.1007/BF02880437
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DOI: https://doi.org/10.1007/BF02880437