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LD50 determination and phenotypic evaluation of three Echeveria varieties induced by chemical mutagens

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Abstract

Objective

The study aims to determine the Lethal Dose 50 (LD50) of Echeveria varieties as induced by chemical mutagens.

Methods

Three cultivated varieties from Echeveria species, namely ‘Brave,’ ‘Viyant,’ and ‘Snow bunny,’ were induced with chemical mutagens: colchicine, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), and sodium azide (NaN3). Each mutagen was diluted to different concentrations: colchicine (0.2%, 0.4%, 0.6%, 0.8%, 1.0%), NaN3 (0.02%, 0.04%, 0.06%, 0.08%, 0.1%), EMS, and MMS (0.1%, 0.2%, 0.3%, 0.4%, 0.5%). Soaking durations for each concentration level were 3, 6, 9, and 12 h. The survival rate and phenotypic data for mutated plants per variety in response to chemical mutagens were collected.

Results

The LD50 evaluation revealed maximum concentration and treatment duration vary per varieties. Regardless of varieties, EMS-treated leaf cuttings had the highest survival rate. However, upon phenotypic evaluation, the results revealed that mutagenic plants were only taken from those treated with colchicine.

Conclusion

The use of colchicine to produce mutated succulents should be further investigated at the molecular level. The results of the study are highly beneficial for mutation breeding programs for other succulent varieties or other related crops.

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References

  1. van Harten AM (1998) Mutation breeding: theory and practical application. Cambridge University Press, Cambridge

    Google Scholar 

  2. International Atomic Energy Agency (IAEA) (2011) IAEA mutant database (International Atomic Energy Agency)

  3. Springer (2008) Physical mutagens. In: Encyclopedia of genetics, genomics, proteomics and informatics. Springer, Dordrecht

  4. Jain S (2010) In vitro mutagenesis in banana (Musa spp.) improvement. Acta Hortic. 879:605–614

    Article  Google Scholar 

  5. Kulkarni VM, Ganapathi TR, Suprasanna P, Bapat VA (2007) In vitro mutagenesis in banana (Musa spp.) using gamma irradiation. In: Jain SM, Häggman H (eds) Protocols for micropropagation of woody trees and fruits. Springer, Dordrecht

    Google Scholar 

  6. Predieri S (2000) Mutation induction and tissue culture in improving fruits. Plant Cell Tiss Org 64:185–210

    Article  Google Scholar 

  7. Broertjes C, Van Harten AM (1988) Developments in Crop Science. Elsevier Publication, Amsterdam

    Google Scholar 

  8. Kondo E, Nakayama M, Kameari N, Tanikawa N, Morita Y, Akita Y, Hase Y, Tanaka A, Ishizaka H (2009) Red-purple flower due to delphinidin 3,5-diglucoside, a novel pigment for Cyclamen spp., generated by ion-beam irradiation. Plant Biotechnol. J. 26:565–569

    Article  CAS  Google Scholar 

  9. Edwards EJ, Ogburn RM (2013) Plant venation: from succulence to succulents. Curr Biol 23:340–341

    Article  Google Scholar 

  10. Sevik H, Karakas H, Karaca U (2013) Color—chlorophyll relationship of some indoor ornamental plants. I.J.E.S.R.T 2:1706–1712

    Google Scholar 

  11. Pathirana R (2011) Plant mutation breeding in agriculture. CAB Rev: Perspect Agric, Vet Sci, Nutr Nat Resour 6:1–20

    Article  Google Scholar 

  12. Szarejko I (2011) In plant mutation breeding and biotechnology. CABI International, Wallingford, pp 387–410

    Google Scholar 

  13. Cabahug RA, Soh SY, Nam SY (2016) Growth of Crassulaceae succulents as influenced by leaf-cutting type and planting position. Flower Res J 24:255–263

    Article  Google Scholar 

  14. Ash A, Ellis B, Hickey LJ, Johnson K, Wilf P, Wing S (1999) Manual of leaf architecture. Smithsonian Institution, Washington, USA

    Google Scholar 

  15. Manuel GL, Chaves L, Ramirez R, Camejo Y (2002) Agricultural yield and internal quality of fruits in tomato plants (Lycopersicon esculentum) from seeds irradiated with x-rays. Alimentaria 40:113–116

    Google Scholar 

  16. Sagnard BB, Fouilloux G, Chupeau Y (1996) Induced albino mutations as a tool for genetic analysis and cell biology in flax (Linum usitatissimum). J Exp Bot 47:189–194

    Article  Google Scholar 

  17. Food and Agriculture Organization (FAO) (2009) FAO/IAEA database of mutant variety and genetic stocks

  18. Jobelius HH, Scharff HD (2012) Hydrazoic acid and azides. In: Ullmann’s encyclopedia of industrial chemistry, vol 18, pp 97–102

  19. Awan MA, Konzak CF, Rutger JN, Nilan RA (1980) Mutagenic effects of sodium azide in rice. Crop Sci 20:663–668

    Article  CAS  Google Scholar 

  20. Talame V, Bovina R, Sanguineti MC, Tuberosa R, Lundqvist U, Salvi S, Tillnore A (2008) Resource for the discovery of chemically induced mutants in barley. Plant Biotechnol J 6:477–485

    Article  CAS  Google Scholar 

  21. Mostafa GG (2011) Effect of sodium azide on the growth and variability induction in Helianthus annuus L. Int J Plant Breed Genet 5:76–85

    Article  Google Scholar 

  22. Abou Dahab AM, Tarek AA, Heika AAM, Taha LS, Gabr AMM, Metwally SA, Awatef IAR (2017) Propagation and chemical mutagenic induction of Eustoma grandiflium plant using tissue culture technique. Asian J Appl Sci Technol 1:496–511

    Google Scholar 

  23. Nilan RA, Sideris EG, Kleinhofs A, Sander C, Konzak CF (1973) Azide a potent mutagen. Mutat Res 17:142–144

    Article  CAS  Google Scholar 

  24. Al Achkar W, Sabatier L, Dutrillaux B (1989) How are sticky chromosomes formed? Ann Genet 32:10–15

    CAS  PubMed  Google Scholar 

  25. Gruszka D, Szarejko I, Maluszynski M (2011) Plant mutation breeding and biotechnology. Food and Agriculture International Atomic Energy Agency, Vienna, Austria, pp 159–166

    Google Scholar 

  26. Castro CM, Oliveria AC, Calvaho FIF (2003) Changes in allele frequencies in colchicine treated ryegrass population with APD marker. Agrociencia 9:107–112

    Google Scholar 

  27. Blakeslee AF, Avery AG (1937) Method of inducing doubling of chromosomes in plants by treatment with colchicine. J. Heredity 28:393–411

    Article  CAS  Google Scholar 

  28. Ning GG, Shi XP, Hu HR, Yan Y, Bao MZ (2009) Development of a range of polyploidy lines and Petunia hybrida and the relationship of ploidy with the single-/double-flower trait. HortScience 44:250–255

    Article  Google Scholar 

  29. El-Nashar YI, Ammar MH (2016) Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L. Genet Mol Res 15:15027745

    Google Scholar 

  30. Kushwah KS, Verma RC, Patel S, Jain NK (2018) Colchicine-induced polyploidy in Chrysanthemum carinatum L. J. Phylogenetics Evol. Biol. 6:1–4

    Article  Google Scholar 

  31. Pickens KA, Chen ZM, Kania SA (2006) Effects of colchicine and oryzalin on callus and adventitious shoot formation of Euphorbia pulchurrima `Winter Rose’. HortSci 41:1651–1655

    Article  CAS  Google Scholar 

  32. Nooden LD (1971) Physiology and developmental effects of colchicine. Plant Cell Physiol 12:759–770

    CAS  Google Scholar 

  33. Mahna SK, Garg R, Parvateesam M (1989) Mutagenic effects of sodium azide in black gram. Curr Sci 58:582–584

    CAS  Google Scholar 

  34. Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A, Rahim HA, Miah G, Usman M (2016) Principle and application of plant mutagenesis in crop improvement: a review. Biotechnol Biotechnol Equip 30(1):1–16

    Article  CAS  Google Scholar 

  35. Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Odden AR, Comai L, Henikoff S (2003) Spectrum of chemically induced mutations from a large scale reverse-genetic screen in Arabidopsis. Genetics 164:731–740

    CAS  PubMed  PubMed Central  Google Scholar 

  36. Arisha MG, Liang BK, Muhammad Shah SN, Gong ZH, Li WD (2014) Kill curve analysis and response of first-generation capsicum annuum L. B12 cultivar to ethyl methanesulfonate. Genet Mol Res 13:10049–10061

    Article  CAS  Google Scholar 

  37. Talebi AB, Talebi AB, Shahrokhifar B (2012) Ethyl methane sulphonate (EMS) induced mutagenesis in Malaysian rice (cv. MR219) for lethal dose determination. Am. J. Plant Sci. 3:1661–1665

    Article  CAS  Google Scholar 

  38. Wang L, Zhang B, Li J, Yang X, Ren Z (2014) Ethyl methanesulfonate (EMS)-mediated mutagenesis of cucumber (Cucumis sativus L.). Agricultural Sciences 5:716–772

    Article  Google Scholar 

  39. Selvaraj R, Jayakumar S (2003) Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonate in sunflower (Helianthus annuus L.). Madras Agric. J. 90:574–576

    Google Scholar 

  40. Bhat TA, Parveen S, Khan AH (2007) Meiotic studies in two varieties of Vicia faba L. (Fabaceae) after EMS treatment. Asian J Plant Sci 6:51–55

    Article  Google Scholar 

  41. Khursheed S, Khan S (2014) Ethyl methanesulphonate (EMS), a potent chemical mutagen: a review. Int J Sci Res 3:448–490

    Google Scholar 

Download references

Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP; Ministry of Science, ICT & Future Planning) (No. 2017R1C1B5017830).

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Author notes

  1. Raisa Aone M. Cabahug and My Khanh Tran Thi Ha have contributed equally to this work.

Authors and Affiliations

  1. Chromosome Research Institute, Sahmyook University, Seoul, 01795, Korea

    Raisa Aone M. Cabahug

  2. Department of Convergence Science, Sahmyook University, Seoul, 01795, Korea

    My Khanh Tran Thi Ha & Yoon-Jung Hwang

  3. Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Korea

    Ki-Byung Lim

  4. Department of Environmental Horticulture, Sahmyook University, Seoul, 01795, Korea

    Raisa Aone M. Cabahug

Authors
  1. Raisa Aone M. Cabahug
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  2. My Khanh Tran Thi Ha
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Correspondence to Yoon-Jung Hwang.

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Raisa Aone M. Cabahug, My Khanh Tran Thi Ha, Ki-Byung Lim, and Yoon-Jung Hwang declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Cabahug, R.A.M., Ha, M.K.T.T., Lim, KB. et al. LD50 determination and phenotypic evaluation of three Echeveria varieties induced by chemical mutagens. Toxicol. Environ. Health Sci. 12, 1–9 (2020). https://doi.org/10.1007/s13530-020-00049-3

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  • DOI: https://doi.org/10.1007/s13530-020-00049-3

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