Skip to main content
SpringerLink
Log in

Studies on mutagen sensitivity, effectiveness and efficiency in bell pepper (Capsicum annuum (L.) var. Grossum)

  • Research Articles
  • Published:
Vegetos Aims and scope Submit manuscript

Abstract

Mutation breeding explores genetic diversity in useful traits to develop new, high-yielding, and improved cultivars. This five year study revealed different mutagenic treatments in bacterial wilt susceptible cultivar of Bell pepper [Capsicum annum (L.)]. Healthy and pure seeds of Bell pepper (California Wonder) were treated with gamma rays (0.5kR, 1kR, 3kR, 5kR, 8kR, 11kR, 13kR, 16kR, 19kR and 22kR) and ethyl methane sulphonate (EMS) (0.01%, 0.25%, 0.5%, 0.75%, 1.0%, 1.25%, 1.5%, 1.75%, 2.0% and 3.0%). A steady reduction in germination and subsequent survival of the treated population, seedling height and pollen fertility were observed with increasing doses/concentrations of mutagens regardless of the mutagens used. Mutagenic effectiveness decreased with increase in mutagen dose in gamma rays (physical mutagen treatments), but the trend was different for EMS which did not follow a regular descending or ascending order. Overall, EMS proved to be more efficient and effective mutagen than gamma irradiation. The coefficient of variation induced by EMS treatments was higher as compared to gamma treatments, suggesting that EMS was more effective than gamma rays. The viable mutants recorded with desirable characters will prove to be of potential value in conventional plant breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adamu AK, Clung SS, Abubakar S (2004) Effects of ionizing radiation (gamma-rays) on tomato (Lycopersicon esculentum L). Nigerian J Expt Appl Bio 5(2):185–193

    Google Scholar 

  • Akhtar N (2014) Effect of physical and chemical mutagens on morphological behavior of Tomato (Solanum Lycopersicum) Cv. “Rio Grande” under heat stress conditions. Plant Breed Seed Sci 70:69–79

    Article  Google Scholar 

  • Alcantra TP, Bosland PW, Smith DW (1996) Ethyl methane sulfonate induced mutagenesis of Capsicum annuum. J Heredity 87:239–241

    Article  Google Scholar 

  • Arisha MH, Shah SNM, Gong ZH, JingH, Li C 1, Zhan HX (2015) Ethylmethane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L). Front plant sci 6:1–11

  • Aslam R, Bhat TM, Choudhary S, Ansari MYK, Shahwar D (2017) Estimation of genetic variability, mutagenic effectiveness and efficiency in M2 flower mutant lines of Capsicum annuum L. treated with caffeine and their analysis through RAPD markers. J King Saud Univ – Sci 29:274–283

    Article  Google Scholar 

  • Awachar SR, Sharma P (2022) Induction of genetic variability in Pusa Jwala and Pusa Sadabahar (Capsicum annuum L.) through mutation via sodium azide and hydroxyl amine. YMER 21(6):313–318

    Article  Google Scholar 

  • Bhat S, Sharma S, Sharma VK (2017) Effect of EMS induced morphological mutation in Strawberry. J Hill Agri 8(1):50–55

    Article  Google Scholar 

  • Blixt S (1972) Mutation Genetics in Pisum. Agri Horti Gen 30:1–293

    Google Scholar 

  • Bolbhat SN, Bhoge VD, Dhumal KN (2012) Induced mutations in horsegram (Macrotyloma uniflorum (Lam.) Verdc): chlorophyll mutations, mutagenic efficiency and effectiveness. Int J Life Sci Pharma Res 2(4):159–168

    Google Scholar 

  • Botticella E, Sestili F, Hernandez-Lopez A, Phillips A, Lafiandra D (2011) High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes. BMC Plant Bio 11:156

    Article  CAS  Google Scholar 

  • Daudu OAY, Falusi OA (2011) Induced genetic variability for morphological and yield parameters in (Capsicum annum and Capsicum frutescens). Waste Manage Bioresource Technol 1:31–37

    Google Scholar 

  • Devi AS, Mullainathan L (2011) Physical and chemical mutagenesis for improvement of chilli (Capsicum annuum L). World Appl Sci J 15(1):108–113

    Google Scholar 

  • Dongfu H, Jianwen HE, Wenting FU, Mingwen HU, Hong Y (2022) Phenotypic variation analysis of Ethyl Methane Sulfonate induced mutant population of pepper. Pak J Bot 54(1):195–203

    Article  Google Scholar 

  • Espina MJ, Ahmed CM, Bernardini A, Adeleke E, Yadegari Z, Arelli P, Pantalone V, Taheri A (2018) Development and phenotypic screening of an Ethyl methane sulfonate mutant population in soybean. Front Plant Sci 9:394

    Article  PubMed  PubMed Central  Google Scholar 

  • Gupta N, Sood S, Sood VK (2017) Spectrum and frequency of mutations induced by gamma rays and EMS in okra [Abelmoschus esculentus (L.) Moench]. Electron J Plant Breed 8(3):967–972

    Article  Google Scholar 

  • Gupta N, Sood S, Sood VK (2018) Induction and characterisation of agronomically useful mutants in okra [Abelmoschus esculentus (L.) Moench]. Agri Res J 55(1):47–51

    Google Scholar 

  • Honda I, Kikuchi K, Matsuo S, Fukuda M, Saito H, Ryuto H et al (2006) Heavy-ion-induced mutants in sweet pepper isolated by M1 plant selection. Euphytica 152(1):61–66

    Article  CAS  Google Scholar 

  • Jabeen N, Mirza B (2004) Ethyl Methane Sulfonate induces morphological mutations in Capsicum annuum. Int J Agri Bio 6(2):340–345

    CAS  Google Scholar 

  • Jegadeesan S, Reddy KS (2021) Radiation-induced mutations in mutation breeding, genetic diversity and crop adaptation to climate change In Mutation breeding, genetic diversity and crop adaptation to climate change. 31: 303–311

  • Jo YD, Kim JB (2019) Frequency and spectrum of radiation-induced mutations revealed by whole-genome sequencing analyses of plants. Quantum Beam Science 3(2):7

    Article  CAS  Google Scholar 

  • Just D, Garcia V, Fernandez L, Bres C, Mauxion JP, Petit J et al (2013) Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato. Plant Biotechnol 30:225–231. https://doi.org/10.5511/plantbiotechnology.13.0622a

    Article  CAS  Google Scholar 

  • Konzak CF (1965) Efficient chemical mutagenesis, in: the use of induced mutations in plant breeding. Report of the FAO/IAEA technical meeting organized by the food and agriculture organization of the United Nations and the International Atomic Energy Agency in cooperation with the European Association for Research on Plant breeding. Pergamon Press, Rome, Italy, pp 49–70

    Google Scholar 

  • Konzak CF, Nilan RA, Wanger J, Foster RJ (1965) Efficient chemical mutagenesis. Rad Bot 5:49–70

    Google Scholar 

  • Kumar G, Tripathi R (2008) Lead-induced cytotoxicity and mutagenicity in grass pea. Turkish J Biol 32:73–78

    CAS  Google Scholar 

  • Kumari V, Chaudhary HK, Prasad R, Kumar A, Singh A, Jambhulkar S, Sanju S (2016) Frequency and spectrum of mutations induced by gamma radiations and Ethyl Methane Sulphonate in Sesame (Sesamum indicum L). Scientia 14(3):270–278

    CAS  Google Scholar 

  • Neeraj, Kumar V (2022) Induced morphological mutants by gamma rays and EMS in chilli (Capsicum annum L). Pharma Innov J 11(5):1306–1309

    Google Scholar 

  • Pathirana R (2012) Plant mutation breeding in agriculture In CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 6(32): 107–126

  • Sikder S, Biswas P, Hazra P, Akhtar S, Chattopadhyay A, Badigannavar AM, D‟Souza SF (2013) Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS. Ind J Gen 73(4):392–399

    Article  Google Scholar 

  • Sikder S, Ravat VK, Basfore S, Hazra P (2015) Isolation of induced mutants using gamma ray and Ethyl Methane Sulphonate in Tomato (Solanum lycopersicum L). Electron J Plant Breed 6(2):464–471

    Google Scholar 

  • Smith HH (1972) Comparative genetic effects of different physical nutagens in higher plants. Induced mutations and plant improvement. International Atomic Energy Agency, Vienna, pp 75–93

    Google Scholar 

  • Sneath PH, Bread RS, Murray EG, Smith RN (1986) Bergey’s manual of determining bacteriology. Williams and Wilkins Co., London, p 232

    Google Scholar 

  • Sood S, Jambhulkar S, Singh Y, Gupta N, Sharma S (2017) Induced chlorophyll mutations in Bell pepper (Capsicum annuum L. var. Grossum). J Appl Nat Sci 9(2):1085–1089

    Article  CAS  Google Scholar 

  • Waghmare VN, Mehra RB (2001) Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Ind J Gen 61:53–56

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Vegetable Science, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya Palampur, Palampur, Himachal Pradesh, India

    Sonia Sood

  2. Faculty of Agriculture, Baba Farid College, Bathinda, Punjab, India

    Nivedita Gupta

Authors
  1. Sonia Sood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nivedita Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nivedita Gupta.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sood, S., Gupta, N. Studies on mutagen sensitivity, effectiveness and efficiency in bell pepper (Capsicum annuum (L.) var. Grossum). Vegetos (2023). https://doi.org/10.1007/s42535-023-00634-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s42535-023-00634-0

Keywords

Search

Navigation