Abstract
The aerobic adaptation of anaerobically grownP. denitrificans carried out under conditions of limited growth is characterized by an exponential decrease of nitrite reductase activity and a sharp increase of cytochrome oxidase and a slow increase of NADH:cytochromec oxidase reductase and succinate dehydrogenase activities. The adaptation in a minimal adaptation medium under conditions of active or blocked protein synthesis showed that in addition to the degradation component of turnover during the aerobic adaptation other degradation enzyme(s), whose synthesis is induced by oxygen, are involved. This degradation system plays an essential role in the rapid disappearance of nitrite reductase and a pronounced decrease of the membranebound cytochromec oxidase activities during aerobic adaptation in the minimal adaptation medium.
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Abbreviations
- PMS:
-
phenazine methosulphate
- DCIP:
-
2,6-dichlorophenolindophenol
- PMSF:
-
phenylmethanesulphonyl fluoride
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Nejedlý, K., Dadák, V. Activities of respiratory enzymes during aerobic adaptation of anaerobically grownParacoccus denitrificans . Folia Microbiol 28, 253–261 (1983). https://doi.org/10.1007/BF02879555
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DOI: https://doi.org/10.1007/BF02879555