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The attempt to transform streptomycin-resistance inMycobacterium phlei

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Abstract

Acid-fast and non-acid-fast strains ofMycobacterium phlei were used for the induction of streptomycin-resistance by isolated DNA. Biological activity of a transforming principle isolated from the cells homogenized in a Hughes press was confirmed. No reproducible positive results were obtained under given experimental conditions. Possible reasons for the lack of positive results are discussed.

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Authors and Affiliations

  1. Department of Genetics and Variability of Microorganisms, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    V. Rytíř, J. Hubáček & I. Málek

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  1. V. Rytíř
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  2. J. Hubáček
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  3. I. Málek
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Rytíř, V., Hubáček, J. & Málek, I. The attempt to transform streptomycin-resistance inMycobacterium phlei . Folia Microbiol 11, 257–262 (1966). https://doi.org/10.1007/BF02878894

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