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Biological decomposition of fulvic acid preparations

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Abstract

Decomposition of preparations of various fractions of fulvio acids in pure cultures of bacteria and in the soil was investigated. In the soils enriched with fulvic acids the amount of bacteria increased, oxygen consumption and formation of carbon dioxide followed a typical sigmoid curve. The above measurements indicated that mineralization occurred after a very short or negligible lag phase. During the decomposition of fulvic acids the ability of microorganisms to oxidize aromatic compounds,e.g. vanillio andphydroxybenzoic acid, increased. The presence of aromatic structures in the used preparations of fulvic acids was demonstrated on the basis of their IR spectra and according to the results of Chromatographic analyses of their hydrolysates. The results indicated a relationship between metabolism offulvio acids and aromatic oompounds. In samples of the soil preincubated with glucose the fulvic acids decomposed more rapidly than in untreated samples.

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References

  • Alexandbova I. V.: On the role of microbial metabolites during the formation of humic substances. RussianPochvovedenije No.8. 71 (1968).

    Google Scholar 

  • Audus L. J.: A new soil perfusion apparatus.Nature 158, 419 (1946).

    Article  CAS  Google Scholar 

  • Bremner J. M., Shaw K.: Denitrification of soil. I. Methods of investigation.J. Agr. Sci. 51, 22 (1958).

    CAS  Google Scholar 

  • Burges N. A.: Biological processes in the decomposition of organic matter. In: E. G. Hallsworth, D. V. Crawford (Eds.):Experimental Pedology. Publ. Butterworths, London, 1964, p. 189.

    Google Scholar 

  • Burges A., Latter P.: Decomposition of humic acid by fungi.Nature 186, 404 (1960).

    Article  CAS  Google Scholar 

  • DeBorger R.: Note sur la détermination du caractére aromatique des acides humiques et fulviques.Compt. Rend. D 269, 1564 (1969).

    CAS  Google Scholar 

  • DeBorger R.: Le dégagement de CO2 lors de la décomposition microbiologique des acides fulviques.Compt. Rend. D 274, 2104 (1972).

    CAS  Google Scholar 

  • Drobník J.: A Warburg vessel for soil samples.Nature 188. 686 (1960).

    Article  Google Scholar 

  • Fedorov M. V., Ilyina T. K.: Utilization of humic acids by soil actinomycetes as the only source of carbon and nitrogen. (In Russian)Mikrobiologiya 32, 272 (1963).

    CAS  Google Scholar 

  • Grunda B.: The effect of humus substances on the count of soil microorganisms in culture.Folia Microbiol. 15, 216 (1970).

    Google Scholar 

  • Hais I. M., Macek K. (Eds.):Paper Chromatography. Publ. House of the Czechoslovak Academy of Sciences, Prague, 1963.

    Google Scholar 

  • Hurst H. M.: Aromatic acid-reducing systems in fungi. In: Pridham J. B. (Ed.):Enzyme Chemistry of Phenolic Compounds. Proceedings of Plant Phenolic Group Symposium. Pergamon Press, London, 1963, p. 121.

    Google Scholar 

  • Hurst H. M., Burges N. A.: Lignin and humic acids. In: McLaren A. D., Peterson G. H. (Eds.):Soil Biochemistry. M. Dekker, New York 1967, p. 260.

    Google Scholar 

  • Hurst H. M., Burges A., Latter P.: Some aspects of the biochemistry of humic acid decomposition by fungi.Phytochemistry 1, 227 (1962).

    Article  CAS  Google Scholar 

  • Jakab T., Dubach P., Mehta N. C., Deuel H.: Abbau von Huminstoffen. I. Hydrolyse mit Wasser und Mineralsäuren.Z. Pflanzenernäh. Düng., Bodenk. 96, 213 (1962).

    Article  Google Scholar 

  • Jansson S. K.: On the establishment and use of tagged microbial tissue in soil organic matter research. 7thIntern. Congt. Soil. Sci., Madison, Wisc., USA, III.25, 635 (1960).

    Google Scholar 

  • Johnston H. H.: Soil organic matter. II. Studies on the origin and chemical structure of soil humic acids.Soil Sci. Soc. Amer. Proc. 25, 32 (1961).

    CAS  Google Scholar 

  • Kononova M. M.:Soil Organic Matter. Pergamon Press, London, 1961, p. 74.

    Google Scholar 

  • Kunc F., Macura J.: Oxidation of aromatic compounds in soil.Folia Microbiol. 11, 248 (1966).

    Article  CAS  Google Scholar 

  • Latter P., Burges A.: Experimental decomposition of humic acid by fungi.7th Intern. Congr. Soil Sci., Madison, Wisc., USA26, 643 (1960).

    Google Scholar 

  • Macura J., Szolnoki J., Kunc F., Vančura V., Babický A.: Decomposition of glucose continuously added to soil.Folia Microbiol. 10, 44 (1965).

    Article  CAS  Google Scholar 

  • Mathur S. P.: Microbial use of podzol Bh fulvic acids.Can. J. Microbiol. 15, 677 (1969).

    PubMed  CAS  Google Scholar 

  • Mathur S. P., Paul E. A.: A microbiological approach to the problem of soil humic acid structures.Nature 212, 646 (1966).

    Article  CAS  Google Scholar 

  • Mishustin E. N., Nikitin D. I.: Decomposition of humic acids by the soil microflora. (In Russian)Mikrobiologiya 30, 841 (1961).

    CAS  Google Scholar 

  • Mortensen J. L., Himes F. L.: Soil organic matter. In: Bear F. E. (Ed.):Chemistry 0f the Soil. Amer. Chem. Soc. Mono 160, Rheinhold Publ. Comp., New York, 1964, p. 224.

    Google Scholar 

  • Mrysha G. N.: Utilization of nitrogen of humic compounds by soil micro-organisms. (In Russian)Izv. Timiryazev. Sel.-Choz. Acad. No. 5, 46 (1966).

    Google Scholar 

  • Mrysha G. N.: Micro-organisms of the genusPseudomonas utilizing humic acids of soils. (In Russian)Izv. Timiryazev. Sel.-Choz. Acad., No.2, 35 (1967).

    Google Scholar 

  • Mrysha G. N.: Micro-organismsof Pseudomonas genus growing on humin compounds. (In Russian)Mikrobiologiya 38, 492 (1969).

    CAS  Google Scholar 

  • Murzakov B. G., Vasilyeva L. V.: Decomposition of fulvic acids fractions by soil microflora. (In Russian)Izv. AN USSR, Ser. Biol., No.10, 906 (1970).

    Google Scholar 

  • Najmr S., Cikánek M.: Simultaneous determination of carbon and nitrogen in soil. (In Czech)Sborník ČSAZV A 26, 285 (1953).

    Google Scholar 

  • Nikitin D. I.: Decomposition of soil humic acids by microorganisms. (In Russian)Izv. AN USSR, Ser. Biol. No.4, 618 (1960).

    Google Scholar 

  • Nikitin D. I., Vasilyeva L. V., Lokhmacheva R. A.: New and rare forms of soil micro organisms. (In Russian) Nauka, Moscow, 1966.

    Google Scholar 

  • Orlov D. S.: Humic acids of soil. (In Russian) Publ. House of Moscow University, Moscow 1974.

    Google Scholar 

  • Shivrina A. N., Rydalevskaya M. D., Tereshenkova I. A.: On aromatic compounds as components of humus acids. (In Russian)Pochvovedenie No.1, 65 (1968).

    Google Scholar 

  • Taylor C. B.: The nutritional requirements of predominant flora of the soil.Proc. Soc. Appl. Bact. 14, 101 (1951).

    Google Scholar 

  • Viljams V. V.: The separation and quantitative determination of soil humic acids. (In Russian)Izv. Timiryazev. Sel.-Choz. Acad. No.2, 126 (1965).

    Google Scholar 

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Kunc, F., Lokhmacheva, R.A. & Macura, J. Biological decomposition of fulvic acid preparations. Folia Microbiol 21, 257–267 (1976). https://doi.org/10.1007/BF02876901

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